Cell penetrating peptide tethered bi-ligand liposomes for delivery to brain in vivo: Biodistribution and transfection
Cell penetrating peptide tethered bi-ligand liposomes for delivery to brain in vivo: Biodistribution and transfection
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DOI：
10.1016/j.jconrel.2013.01.016
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发表时间：
2013-04-10
影响因子：
10.8
通讯作者：
Singh, Jagdish
中科院分区：
文献类型：
--
作者：
Sharma, Gitanjali;Modgil, Amit;Singh, Jagdish
靶向纳米颗粒系统在跨越血脑屏障(BBB)传递治疗药物方面具有非凡的潜力。在这项工作中，我们研究了新型双配体(转铁蛋白-聚-L-精氨酸)脂质体载体在体内将所需基因输送到脑内的可能性。对载体的体内评价是临床翻译的关键。我们遵循了一种结合转铁蛋白受体靶向和增强细胞穿透性的创新方法来设计脂质体载体，以改善分子进入大脑的运输。研究了1，1‘-二十八烷基-3，3，3’，3‘-四甲基吲哚碳菁(DIR)标记脂质体在成年大鼠体内的生物分布。我们发现双配体脂质体在大鼠脑内的蓄积显著(p<0.05)。
Targeted nano-particulate systems hold extraordinary potential for delivery of therapeutics across blood brain barrier (BBB). In this work, we investigated the potential of novel bi-ligand (transferrin-poly-L-arginine) liposomal vector for delivery of desired gene to brain, in vivo. The in vivo evaluation of the delivery vectors is essential for clinical translation. We followed an innovative approach of combining transferrin receptor targeting with enhanced cell penetration to design liposomal vectors for improving the transport of molecules into brain. The biodistribution profile of 1, 1'-dioctadecyl-3,3,3', 3'-tetramethyl-indocarbocyanine iodide(DiR)-labeled liposomes was evaluated in adult rats after single intravenous injection at dose of 15.2 mu moles of phospholipids/kg body weight. We demonstrated that bi-ligand liposomes accumulated in rat brain at significantly (p