Clomazone influence soil microbial community and soil nitrogen cycling
Clomazone influence soil microbial community and soil nitrogen cycling
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异恶草酮影响土壤微生物群落和土壤氮循环
DOI：
10.1016/j.scitotenv.2018.06.214
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发表时间：
2018-12-10
影响因子：
9.8
通讯作者：
Zheng, Yongquan
中科院分区：
文献类型：
--
作者：
Du, Pengqiang;Wu, Xiaohu;Zheng, Yongquan
通过室内围隔实验，研究了长期暴露于广灭灵（clomazone）对土壤微生物群落及其氮循环功能的影响。将异恶草酮以0.8（推荐剂量）、8和80 mg kg（-1）土壤干重3种浓度施于中国两种典型土壤，培养90 d。定期取出样品，用几种技术进行测定。异恶草酮在本实验中的半衰期为11-126 d。结果仅对最高异恶草酮浓度有显著性。16 S和18 S rDNA基因的下一代测序显示，细菌多样性显着下降，而真菌丰度增加后60天，但没有检测到的影响，对微生物群落。系统聚类和主坐标分析表明，细菌群落结构受到了负面影响。效应量的线性判别分析确定鞘氨醇单胞菌属和节杆菌属为优势菌种。最后，我们测量了土壤NH 4+和NO3-浓度，并使用实时PCR分析了氮循环基因，nifH和amoA的丰度。在前30天，NO3--N含量和氨氧化细菌数量增加。固氮菌在60天后被抑制，但NH 4 +-N浓度保持不变，可能是由氨化作用提供的。(c)2018 Elsevier B. V.版权所有。
We designed an indoor mesocosm experiment to investigate the long-term effects of exposure to clomazone, a widely used herbicide, on soil microbial communities and their nitrogen (N) cycling functions. Clomazone was applied to two typical soils from China at three concentrations: 0.8 (the recommended dosage), 8 and 80 mg kg(-1) soil dry weight, and the mix was incubated for 90 days. Samples were removed periodically for assay with several techniques. The half-lives of clomazone in this experiment were 11-126 d. Results were significant only for the highest clomazone concentration. Next-generation sequencing of the 16S and 18S rDNA genes revealed that bacterial diversity significantly decreased whereas fungal abundance increased after day 60 but with no detectable effect on the microbial community. Hierarchical cluster and principal coordinates analysis revealed that the bacterial community structure was negatively impacted. Linear discriminant analysis of effect size identified Sphingomonas and Arthrobacter as the predominant bacterial species. Finally, we measured soil NH4+ and NO3- concentrations and used real-time PCR to analyze the abundance of the N-cycling genes, nifH and amoA. In the first 30 days, the NO3--N content and the number of ammonia-oxidizing bacteria increased. N-2-fixing bacteria were inhibited after 60 days, but the NH4+-N concentration remained unchanged and was likely provided by ammoniation. (c) 2018 Elsevier B.V. All rights reserved.