Enhanced salt tolerance in tomato plants constitutively expressing heat-shock protein in the endoplasmic reticulum
Enhanced salt tolerance in tomato plants constitutively expressing heat-shock protein in the endoplasmic reticulum
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DOI：
10.4238/gmr.15028301
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发表时间：
2016-01-01
影响因子：
0.4
通讯作者：
Liu, J.
中科院分区：
文献类型：
--
作者：
Fu, C.;Liu, X. X.;Liu, J.
内质网 (ER) 中未折叠或错误折叠蛋白的积累会导致 ER 应激并激活未折叠蛋白反应 (UPR) 信号通路。 UPR 信号通路与植物对不利环境条件的反应有关。因此，UPR信号通路的变化可能会影响植物的非生物耐受性。本研究探讨了内质网小热休克蛋白（ER-sHSP）在提高植物抗盐胁迫方面的作用。在盐胁迫条件下，与野生型非转基因植物相比，ER-sHSP转基因植物具有更旺盛的根系，保持更高的相对含水量，吸收更少的Na+，积累更多的渗透剂和Ca2+，并且对光系统的损害更小。此外，我们发现盐胁迫下 ER-sHSP 的组成型表达抑制了其他 ER 分子伴侣的表达。这些结果表明，ER-sHSP的组成型表达显着增强了番茄植株的耐盐性，减轻了植物细胞内盐胁迫引起的ER胁迫。
The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and activates the unfolded protein response (UPR) signaling pathway. The UPR signaling pathway is associated with plant responses to adverse environmental conditions. Thus, changes in the UPR signaling pathway might affect plant abiotic tolerance. Here, the role of ER small heat-shock protein (ER-sHSP) in improving plant resistance to salt stress was explored. Under salt stress conditions, ER-sHSP transgenic plants were found to have more vigorous roots, maintain a higher relative water content, absorb less Na+, accumulate more osmolytes and Ca2+, and sustain less damage to the photosystem, compared to wild-type non-transgenic plants. Furthermore, we found that the constitutive expression of ER-sHSP under salt stress depressed the expression of other ER molecular chaperones. These results indicate that the constitutive expression of ER-sHSP enhanced salinity tolerance of tomato plants significantly, and alleviated the ER stress caused by the salt stress in plant cells.