Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Midgut Response to BmNPV in Susceptible and Near-Isogenic Resistant Strains.
Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Midgut Response to BmNPV in Susceptible and Near-Isogenic Resistant Strains.
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家蚕（鳞翅目）幼虫中肠对敏感菌株和近等基因抗性菌株中 BmNPV 反应的比较转录组分析
DOI：
10.1371/journal.pone.0155341
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Fei DQ
中科院分区：
文献类型：
--
作者：
Wang XY;Yu HZ;Geng L;Xu JP;Yu D;Zhang SZ;Ma Y;Fei DQ
家蚕核型多角体病毒（BmNPV）是造成蚕业严重经济损失的主要病原之一。然而，家蚕对BmNPV的抗性的分子机制仍不清楚。在此，将轮回亲本P50（敏感株）和近等基因系BC 9（抗性株）用于比较转录组研究，检查对BmNPV感染的响应。从两种不同的耐药菌株中共获得了14，300个unigenes;其中，在比较四个转录组后鉴定了869个差异表达基因（DEG）。许多与蛋白质代谢、细胞骨架和凋亡相关的DEG可能参与宿主对BmNPV感染的应答。此外，一些免疫相关基因也被BmNPV感染后改变。具体而言，在去除遗传背景和单个免疫应激反应基因后，发现22个基因可能参与抑制BmNPV感染。这些基因与转运、病毒复制、细胞内天然免疫和细胞凋亡有关。本研究对家蚕抗BmNPV感染的分子机制进行了综述，为今后家蚕抗BmNPV的防治奠定了基础。
Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens causing severe economic losses in sericulture. However, the molecular mechanism of silkworm resistance to BmNPV remains largely unknown. Here, the recurrent parent P50 (susceptible strain) and the near-isogenic line BC9 (resistance strain) were used in a comparative transcriptome study examining the response to infection with BmNPV. A total of 14,300 unigenes were obtained from two different resistant strains; of these, 869 differentially expressed genes (DEGs) were identified after comparing the four transcriptomes. Many DEGs associated with protein metabolism, cytoskeleton, and apoptosis may be involved in the host response to BmNPV infection. Moreover, some immunity related genes were also altered following BmNPV infection. Specifically, after removing genetic background and individual immune stress response genes, 22 genes were found to be potentially involved in repressing BmNPV infection. These genes were related to transport, virus replication, intracellular innate immune, and apoptosis. Our study provided an overview of the molecular mechanism of silkworm resistance to BmNPV infection and laid a foundation for controlling BmNPV in the future.