Effect of oxidized dextran on the stability of gallic acid-modified chitosan-sodium caseinate nanoparticles
Effect of oxidized dextran on the stability of gallic acid-modified chitosan-sodium caseinate nanoparticles
复制标题
氧化右旋糖酐对没食子酸修饰壳聚糖·酪蛋白酸钠纳米粒稳定性的影响
DOI：
10.1016/j.ijbiomac.2021.09.209
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发表时间：
2021-10-10
影响因子：
8.2
通讯作者：
Lu, Yingjian
中科院分区：
文献类型：
--
作者：
Shen, Dongyan;Hu, Qiaobin;Lu, Yingjian
我们将氧化葡聚糖 (Odex) 掺入由没食子酸改性壳聚糖 (GA-CS) 和酪蛋白酸钠 (NaCas) 组成的纳米颗粒中。通过优化GA-CS与NaCas的质量比以及反应溶液的pH值，获得了性能优异、稳定性好的纳米粒子。通过傅里叶变换红外光谱（FT-IR）和荧光光谱仪证实了各种纳米材料之间的相互作用。优化后的复合纳米颗粒直径约为131.2 nm，多分散指数（PDI）为0.14，zeta电位为26.2 mV。我们的结果表明，Odex 增强了 GA-CS/NaCas 纳米颗粒 (NP) 的稳定性和功能。当姜黄素负载量为 10% 时，Odex 交联的 GA-CS/NaCas (NP (Odex)) 的包封率为 96.2%，而未交联的纳米颗粒的包封率为 66.9%。与模拟胃肠液中游离姜黄素的突释曲线相比，观察到封装姜黄素的持续释放曲线。自由基清除测定证实，由于壳聚糖主链上接枝了酚酸，纳米颗粒本身具有优异的抗氧化活性。总体而言，NP（Odex）具有良好的胃肠道稳定性和抗氧化活性，有望用于疏水性生物活性剂的口服递送。
We incorporated oxidized dextran (Odex) into nanoparticles composed of gallic acid-modified chitosan (GA-CS) and sodium caseinate (NaCas). The mass ratio of GA-CS to NaCas and the pH of the reaction solution were optimized to obtain nanoparticles with excellent performance and stability. The interactions among various nanomaterials were confirmed by Fourier-transform infrared spectroscopy (FT-IR) and fluorescence spectrometer. The optimized complex nanoparticles had a diameter of approximately 131.2 nm with a polydispersity index (PDI) of 0.14, and a zeta potential of 26.2 mV. Our results showed that Odex enhanced the stability and function of GA-CS/NaCas nanoparticles (NP). At a curcumin loading of 10%, the encapsulation efficiency of Odex-crosslinked GA-CS/NaCas (NP (Odex)) was 96.2%, whereas that for uncrosslinked nanoparticles was 66.9%. Compared to the burst release profile of free curcumin in simulated GI fluids, the sustained release profile of encapsulated curcumin was observed. Radical-scavenging assays confirmed that the nanoparticles had excellent antioxidant activity themselves due to the grafting of phenolic acid on chitosan backbone. Overall, NP (Odex) with good GI stability and antioxidant activity hold promising for the oral delivery of hydrophobic bioactives.