RFLP analyses of early-maturing European maize germ plasm
RFLP analyses of early-maturing European maize germ plasm
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早熟欧洲玉米种质的 RFLP 分析
DOI：
10.1007/bf00232964
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发表时间：
1992
影响因子：
5.4
通讯作者：
E. Brunklaus
中科院分区：
文献类型：
--
作者：
M. Messmer;A. E. Melchinger;J. Boppenmaier;R. Herrmann;E. Brunklaus
以30个代表欧洲早熟玉米优良种质的自交系为材料，研究了玉米自交系的遗传多样性。用203个克隆-酶组合（106个DNA克隆用限制性内切酶EcoR 1和HindIII）测定了RFLP。遗传材料包括14个燧石，12个凹痕，和4个杂起源线。目的是（1）在这些材料的RFLPs的遗传多样性的特点，（2）比较内和燧石和凹痕杂种优势群之间发现的遗传多样性水平，和（3）检查的有用性的RFLPs分配杂种优势群的自交系。所有的DNA克隆，但两个产生多态性与至少一种限制性内切酶。共有82个和121个克隆酶组合给出了单带和多带的RFLP模式，分别与3.9和7.7 RFLP模式，每个克隆酶组合在所有30个自交系，分别。根据RFLP数据估计的Dice相似系数，各品系间的遗传相似性（GS）在无关的自交系间表现出相当大的变异（0.32 ~ 0.58）。类型燧石x凹痕（0.41）的线组合的平均GS是显着小于无关的燧石线（0.46）和凹痕线（0.46），但有相当大的变化，GS估计每个组内的个别线组合。聚类和主坐标分析的基础上GS值导致单独的组火石和凹痕线，根据系统发育信息。定位线的杂项起源一般是一致的预期的基础上已知的育种行为和谱系。本研究的结果证实了RFLP数据可用于确定自交系的杂种优势群和揭示自交系间的系谱关系。
Thirty inbred lines representing a wide range of early-maturing European elite germ plasm of maize (Zea maysL.) were assayed for RFLPs using 203 clone-enzyme combinations (106 DNA clones with restriction enzymesEcoR1 andHindIII). The genetic materials comprised 14 flint, 12 dent, and 4 lines of miscellaneous origin. Objectives were to (1) characterize the genetic diversity for RFLPs in these materials, (2) compare the level of genetic diversity found within and between the flint and the dent heterotic groups, and (3) examine the usefulness of RFLPs for assigning inbreds to heterotic groups. All but two DNA clones yielded polymorphism with at least one restriction enzyme. A total of 82 and 121 clone-enzyme combinations gave single-banded and multiple-banded RFLP patterns, respectively, with an average of 3.9 and 7.7 RFLP patterns per clone-enzyme combination across all 30 inbreds, respectively. Genetic similarity (GS) between lines, estimated from RFLP data as Dice's similarity coefficient, showed considerable variation (0.32 to 0.58) among unrelated inbreds. The mean GS for line combinations of type flint x dent (0.41) was significantly smaller than for unrelated flint lines (0.46) and dent lines (0.46), but there was considerable variation in GS estimates of individual line combinations within each group. Cluster and principal coordinate analyses based on GS values resulted in separate groupings of flint and dent lines in accordance with phylogenetic information. Positioning of lines of miscellaneous origin was generally consistent with expectations based on known breeding behavior and pedigrees. Results from this study corroborated that RFLP data can be used for assigning inbreds to heterotic groups and revealing pedigree relationships among inbreds.
基于应变测量的PC钢材料蠕变系数评估方法
DOI：
--
发表时间：
2013
期刊：
影响因子：
--
作者：
小川由布子;亀田昭一;佐藤良一;上谷康晴
通讯作者：
上谷康晴