The role of lipopolysaccharide in infectious bone resorption of periapical lesion
The role of lipopolysaccharide in infectious bone resorption of periapical lesion
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DOI：
10.1111/j.0904-2512.2004.00045.x
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发表时间：
2004-03-01
影响因子：
3.3
通讯作者：
Wang, JS
中科院分区：
文献类型：
--
作者：
Hong, CY;Lin, SK;Wang, JS
背景：研究脂多糖（LPS）在根尖周病变诱导的骨吸收中的作用。方法：从两种常见的牙髓致病菌中分离的脂多糖（Fusobacteriumnucleatum和Porphyromonasendodontalis）刺激小鼠巨噬细胞（J774）释放白细胞介素1 α（IL 1 α）和肿瘤坏死因子α（TNF-alpha），并呈时间依赖性。PMB显著抑制这些作用。LPS还刺激J774中基质金属蛋白酶-1（MMP-1）基因表达，而抗IL-1 α和抗TNF-α抗体以及PMB减弱了这种作用。建立Wistar大鼠根尖周病模型。PMB的管理减少了76%至约80%的病变相关的骨吸收的程度，并同时减少了MMP-1-producing macrophages.CONCLUSIONS：这表明，从感染的根管释放的LPS触发IL-1 α和TNF-α的巨噬细胞的合成。这些促炎细胞因子通过巨噬细胞上调MMP-1的产生以促进根尖周骨吸收。
BACKGROUND: The role of lipopolysaccharide (LPS) in periapical lesion-induced bone resorption was investigated. Polymyxin B (PMB), a specific inhibitor of LPS, was evaluated to treat the apical lesion.METHODS: Lipopolysaccharide isolated from two common endodontic pathogens, Fusobacterium nucleatum and Porphyromonas endodontalis, stimulated mouse macrophage (J774) to release interleukin-1alpha (IL-1alpha) and tumor necrosis factor-alpha (TNF-alpha) in a time-dependent manner.RESULTS: Combination of LPS further enhanced the stimulation. PMB inhibited these effects significantly. LPS also stimulated matrix metalloproteinase-1 (MMP-1) gene expression in J774, whereas anti-IL-1alpha and anti-TNF-alpha antibodies, as well as PMB, diminished this effect. A disease model of periapical lesion was established in Wistar rat. Administration of PMB reduced the extent of lesion-associated bone resorption by 76% to approximately 80%, and simultaneously reduced the numbers of MMP-1-producing macrophages.CONCLUSIONS: It is suggested that LPS released from the infected root canal triggers the synthesis of IL-1alpha and TNF-alpha from macrophages. These pro-inflammatory cytokines up-regulate the production of MMP-1 by macrophages to promote periapical bone resorption.