Poor induction of interferon-induced 2′,5′-oligoadenylate synthetase (2–5 AS) in cells persistently infected with mumps virus is caused by decrease of STAT-1α
Poor induction of interferon-induced 2′,5′-oligoadenylate synthetase (2–5 AS) in cells persistently infected with mumps virus is caused by decrease of STAT-1α
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持续感染腮腺炎病毒的细胞中干扰素诱导的 2,5-寡腺苷酸合成酶 (2-5 AS) 的诱导不良是由于 STAT-1α 减少所致
DOI：
10.1007/s007050050434
复制
发表时间：
1998
影响因子：
2.7
通讯作者：
N. Fujii
中科院分区：
文献类型：
--
作者：
N. Yokosawa;T. Kubota;N. Fujii
与未感染的细胞相比，在腮腺炎病毒持续感染的细胞中，干扰素诱导的2′，5 ′-寡腺苷酸合成酶（2-5AS）的诱导较差。至于干扰素（IFN）受体的数量和IFN调节因子（IRF-1和IRF-2）mRNA的水平，两者之间的差异不大。因此，IFN-α信号系统在持续感染细胞中是无效的。研究了腮腺炎病毒持续感染的人羊膜（FL）、人鼻咽癌（KB）、人T淋巴细胞（HUT 78）和人B淋巴细胞（Akata）中干扰素刺激基因因子3α（ISGF-3α）的组成成分STAT-1α（p91）和STAT-2（p113）。STAT-1α，而不是STAT-2，在这些持续感染的细胞中消失，并且该因子不能通过用IFN处理这些细胞而恢复。而持续感染和未感染的对照细胞中STAT-1α mRNA转录水平无差异。因此，我们有理由推断，2-5AS活性的弱诱导是由于STAT-1α的减少，这与IFN-信号转导途径有关。此外，在持续感染腮腺炎病毒的细胞中，其他IFN刺激基因（dsRNA活化蛋白激酶、PKR和MxA蛋白）的诱导也减少。
SummaryPoor induction of interferon-induced 2′,5′-oligoadenylate synthetase (2-5AS) has been demonstrated in cells persistently infected with mumps virus as compared with uninfected cells. As for the number of interferon (IFN) receptors and the level of IFN regulatory factors (IRF-1 and IRF-2) mRNAs, there was little difference between them. Therefore, it is suggested that the IFN-α signaling system is ineffective in the persistently infected cells. Components of IFN-stimulating gene factor 3α (ISGF-3α), STAT-1α (p91) and STAT-2 (p113), were investigated in human amnion (FL), human nasopharyngeal cancer (KB), human T-lymphoid (HUT 78), and human B-lymphoid (Akata) cells persistently infected with mumps virus. STAT-1α, but not STAT-2, disappeared in these persistently infected cells, and this factor was not restored by treatment of these cells with IFN. However, no difference was observed between the levels of STAT-1α mRNA transcript in persistently infected and uninfected control cells. It is reason- able to infer that the poor induction of 2-5AS activity is due to the decrease of STAT-1α in correlation with the IFN-signal transduction pathway. Furthermore, induction of other IFN-stimulated genes (ds-RNA activated protein kinase, PKR, and MxA protein) was also reduced in the cells persistently infected with mumps virus.
腺病毒 E1A 癌基因抑制干扰素信号转导途径。
DOI：
10.1073/pnas.88.18.7913
发表时间：
1991
影响因子：
11.1
作者：
Gutch,MJ;Reich,NC
通讯作者：
Reich,NC
影响因子：
56.9
作者：
SCHINDLER, C;SHUAI, K;DARNELL, JE
通讯作者：
DARNELL, JE