THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE NONTOXIC COMPONENT OF CLOSTRIDIUM-BOTULINUM TYPE-E PROGENITOR TOXIN
THE COMPLETE NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE NONTOXIC COMPONENT OF CLOSTRIDIUM-BOTULINUM TYPE-E PROGENITOR TOXIN
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DOI：
10.1099/00221287-139-1-79
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发表时间：
1993-01-01
期刊：
影响因子：
--
通讯作者：
OGUMA, K
中科院分区：
文献类型：
--
作者：
FUJII, N;KIMURA, K;OGUMA, K
We have analysed the genes borne on a 6.0 kb HindIII fragment cloned from the chromosome of Clostridium botulinum type E strain Mashike. This fragment, cloned within plasmid pU9EMH, contains part of the structural gene for botulinum toxin type E neurotoxin as well as the entire structural gene for a nontoxic component of botulinum type E progenitor neurotoxin gene, ent-120. ent-120 is transcribed in the same direction as the neurotoxin gene and consists of one open reading frame encoding 1162 amino acid residues. Western blotting with anti-nontoxic component sera demonstrates that ent-120 encodes a protein of 120 kDa which forms part of the nontoxic component. ent-120 is homologous to an analogous gene found in botulinum type C strains (69.3% identity at the nucleotide level and 56.1 % at the amino acid level). Two stretches of amino acids at the N-terminus of the ent-120 protein are highly homologous to amino acid sequences within the type E neurotoxin. The stop codon of the ent-120 gene is situated 27 nucleotides upstream from the start codon of the neurotoxin gene.
