Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545
Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545
复制标题
接合穿梭转座子 Tn1545 末端的核苷酸序列
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
P. Courvalin
中科院分区：
文献类型：
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作者：
F. Caillaud;P. Courvalin
摘要：肺炎链球菌的共轭穿梭转座子Tn1545在自身转移后可在革兰氏阳性菌属中转座，克隆后可在大肠杆菌中转座。通过DNA杂交和测序对插入元件前和切除元件后的连接片段和目标的分析表明，Tn1545(1)没有直接或相反方向的末端重复序列，(2)以不对称的方式由末端可变碱基对（1在元件的左侧，3在右侧）插入目标DNA一致序列，(3)插入目标DNA一致序列。(4)在插入时不会产生目标DNA的复制，(5)精确地切除。
SummaryThe conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae transposes in various gram-positive bacterial genera following self-transfer and in Escherichia coli after cloning. Analysis of the junction fragments and of the targets before insertion and after excision of the element by DNA hybridization and sequencing indicated that Tn1545 (1) is not flanked by terminal repeated sequences in either direct or opposite orientation, (2) is flanked, in an asymmetric fashion, by terminal variable base pairs, one at the left and three at the right of the element, (3) inserts in a target DNA consensus sequence, (4) does not generate duplication of the target DNA upon insertion, and (5) excises precisely.