Enhancement of Pyruvate Productivity in Candida glabrata by Deleting the CgADE13 Gene to Improve Acid Tolerance
Enhancement of Pyruvate Productivity in Candida glabrata by Deleting the CgADE13 Gene to Improve Acid Tolerance
复制标题
通过删除 CgADE13 基因提高光滑念珠菌的丙酮酸生产力以提高耐酸能力
DOI：
10.1007/s12257-018-0201-0
复制
发表时间：
2018
影响因子：
3.2
通讯作者：
Liu Liming
中科院分区：
文献类型：
--
作者：
Chen Xiulai;Luo Qiuling;Liu Jia;Liu Liming
耐酸性是衡量工业生产菌株，特别是有机酸生产菌质量的关键因素之一。为了解决这一问题，本研究通过删除光滑假丝酵母中腺苷酸琥珀酸裂解酶（CgADE 13）基因，研究了腺苷酸琥珀酸裂解酶在AMP代谢中的生理功能。在pH4.0时，CgADE 13基因的缺失使菌体的生物量和细胞活力分别比野生型菌株（wt）提高了68.3%和112.0%。此外，CgADE 13缺失还保护了细胞形态，并抑制了ROS的产生。Cgade 13 Δ细胞内ATP含量降低25.0%，H+-ATP酶活性提高15.0%。最后，在30 L间歇式生物反应器中，在pH4.0条件下，Cgade 13 Δ的丙酮酸产量达到53.9 g/L，比野生型提高了166.7%。这是关于C的公差工程的第一个报告。glabrata用于提高丙酮酸生产率，这为代谢工程实现其他化学品的工业化生产提供了一个很好的起点。
Acid tolerance is one of the critical factors to evaluate the quality of the industrial production strains, especially organic acid producing microorganisms. To circumvent this problem, we investigated the physiological function of adenylosuccinate lyase in AMP metabolism fromCandida glabrataby deleting the corresponding gene,CgADE13. At pH 4.0,CgADE13deletion resulted in a 68.3% and 112.0% increase in biomass and cell viability compared to those of wild type strain (wt), respectively. In addition,CgADE13deletion also protected cell morphology and counteracted ROS production. Further, the intracellular ATP level of strainCgade13Δ was decreased by 25.0%, and its H+-ATPase activity was increased by 15.0%. Finally, pyruvate production with strainCgade13Δ in a 30-L batch bioreactor at pH 4.0 reached 53.9 g/L, and pyruvate productivity was increased by 166.7% compared to that of wt. This is the first report regarding tolerance engineering ofC. glabratafor enhancing pyruvate productivity, which provides a good starting point for metabolic engineering to achieve the industrial production of other chemicals.