Membrane receptor-mediated mechano-transduction maintains cell integrity during pollen tube growth within the pistil
Membrane receptor-mediated mechano-transduction maintains cell integrity during pollen tube growth within the pistil
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DOI：
10.1016/j.devcel.2021.02.030
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发表时间：
2021-04-05
影响因子：
11.8
通讯作者：
Yang, Zhenbiao
中科院分区：
文献类型：
--
作者：
Zhou, Xiang;Lu, Jun;Yang, Zhenbiao
侵入性或渗透性生长对发育和生殖过程至关重要（例如，雌蕊的花粉管穿透）和疾病进展（例如，癌转移和真菌菌丝侵入）。侵入或穿透细胞经历来自周围环境的机械压力的剧烈变化，并且必须平衡生长与细胞完整性。在这里，我们表明，拟南芥花粉管的感觉和/或响应机械变化，通过细胞表面受体激酶佛纸密封1（BUPS 1），而出现压缩女性组织。BUPS 1缺陷型花粉管从这些组织中出现后不能保持细胞完整性。BUPS 1的机械转导功能是通过模拟体内生长途径的机械特征的微流控通道装置建立的，基于BUPS 1的机械转导激活来自植物1的Rho样GT3（ROP 1）GT3，以促进胞吐作用，从而促进BUPS 1的配体的分泌，用于机械信号放大和花粉管中细胞壁的硬化。这些发现揭示了细胞基于膜受体的机械传导系统，以科普侵入性或渗透性生长期间的物理挑战。
Invasive or penetrative growth is critical for developmental and reproductive processes (e.g., pollen tube penetration of pistils) and disease progression (e.g., cancer metastasis and fungal hyphae invasion). The invading or penetrating cells experience drastic changes in mechanical pressure from the surroundings and must balance growth with cell integrity. Here, we show that Arabidopsis pollen tubes sense and/or respond to mechanical changes via a cell-surface receptor kinase Buddha's Paper Seal 1 (BUPS1) while emerging from compressing female tissues. BUPS1-defective pollen tubes fail to maintain cell integrity after emergence from these tissues. The mechano-transduction function of BUPS1 is established by using a microfluidic channel device mimicking the mechanical features of the in vivo growth path. BUPS1-based mechano-transduction activates Rho-like GTPase from Plant 1 (ROP1) GTPase to promote exocytosis that facilitates secretion of BUPS1's ligands for mechanical signal amplification and cell wall rigidification in pollen tubes. These findings uncover a membrane receptor-based mechano-transduction system for cells to cope with the physical challenges during invasive or penetrative growth.