Transposase titration in Drosophila melanogaster: a model of cytotype in the P-M system of hybrid dysgenesis.
Transposase titration in Drosophila melanogaster: a model of cytotype in the P-M system of hybrid dysgenesis.
复制标题
果蝇中的转座酶滴定：杂交发育不全 P-M 系统中的细胞型模型。
DOI：
10.1073/pnas.82.23.8119
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Bucholz,LM
中科院分区：
文献类型：
--
作者：
Simmons,MJ;Bucholz,LM
在黑腹果蝇（Drosophilamelanogaster）杂种发育不全的P-M系统中，有些M品系具有染色体P元件。从这些伪M菌株之一的染色体减少了不稳定性的P元素插入突变的烧焦的刚毛位点。我们认为，这种减少表明转座酶与假M株染色体上的P元件以及烧焦基因座上的P元件结合，从而竞争转座酶;因此，可以说假M株的P元件滴定了转座酶，降低了其与烧焦基因座上P元件相互作用的可用性。我们推测在D.虽然在这种情况下，我们提出滴定元件是染色体外的，它们是由转座酶本身的作用产生的。
In the P-M system of hybrid dysgenesis of Drosophila melanogaster, some M strains possess chromosomal P elements. The chromosomes from one of these pseudo-M strains reduced the instability of a P element-insertion mutation of the singed bristle locus. We suggest that this reduction is an indication of competition for a transposase that binds to the P elements on the chromosomes from the pseudo-M strain as well as to the P elements at the singed locus; the pseudo-M strain's P elements might therefore be said to titrate the transposase, reducing its availability to interact with the P elements at the singed locus. We hypothesize that a similar mechanism regulates the movement of P elements in the P strains of D. melanogaster, although in this case we propose that the titrating elements are extrachromosomal and that they are generated by the action of the transposase itself.