Transcriptome analysis in different rice cultivars provides novel insights into desiccation and salinity stress responses.
Transcriptome analysis in different rice cultivars provides novel insights into desiccation and salinity stress responses.
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DOI：
10.1038/srep23719
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发表时间：
2016-03-31
影响因子：
4.6
通讯作者：
Jain M
中科院分区：
文献类型：
--
作者：
Shankar R;Bhattacharjee A;Jain M
干旱和盐碱是影响水稻产量的主要环境因子。耐性水稻品种和敏感水稻品种之间的比较转录组分析可以提供对这些逆境反应的调控机制的深入了解。在本研究中，耐旱性水稻品种[Nagina 22(N22)]和耐盐性水稻品种(Pokkali)与IR64(感病品种)的转录本比较发现，在对照和胁迫条件下，转录反应不同。在逆境条件下，共有801个转录本和507个转录本分别在N22和Pokkali水稻品种中唯一差异表达。基因本体论分析表明，耐逆水稻品种中与非生物胁迫反应相关的转录本的丰富和基因表达的调控。N22的NAC和DBP转录因子家族以及Pokkali的bHLH和C2H2转录因子家族的大量转录产物分别在干旱和盐分胁迫下表现出不同的调控。编码硫氧还蛋白的转录本在N22中高表达，而参与蜡类和萜类代谢的转录本在Pokkali中高表达。总之，本研究中发现的常见和特定品种的逆境反应转录本可以作为发掘水稻非生物逆境耐受性的新候选基因的有用资源。
Drought and salinity are the major environmental factors that affect rice productivity. Comparative transcriptome analysis between tolerant and sensitive rice cultivars can provide insights into the regulatory mechanisms involved in these stress responses. In this study, the comparison of transcriptomes of a drought-tolerant [Nagina 22 (N22)] and a salinity-tolerant (Pokkali) rice cultivar with IR64 (susceptible cultivar) revealed variable transcriptional responses under control and stress conditions. A total of 801 and 507 transcripts were exclusively differentially expressed in N22 and Pokkali rice cultivars, respectively, under stress conditions. Gene ontology analysis suggested the enrichment of transcripts involved in response to abiotic stress and regulation of gene expression in stress-tolerant rice cultivars. A larger number of transcripts encoding for members of NAC and DBP transcription factor (TF) families in N22 and members of bHLH and C2H2 TF families in Pokkali exhibited differential regulation under desiccation and salinity stresses, respectively. Transcripts encoding for thioredoxin and involved in phenylpropanoid metabolism were up-regulated in N22, whereas transcripts involved in wax and terpenoid metabolism were up-regulated in Pokkali. Overall, common and cultivar-specific stress-responsive transcripts identified in this study can serve as a helpful resource to explore novel candidate genes for abiotic stress tolerance in rice.