Consolidated bioprocessing of lignocellulose by a microbial consortium
Consolidated bioprocessing of lignocellulose by a microbial consortium
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DOI：
10.1039/c3ee41753k
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发表时间：
2014-04-01
影响因子：
32.5
通讯作者：
Studer, Michael Hanspeter
中科院分区：
文献类型：
--
作者：
Brethauer, Simone;Studer, Michael Hanspeter
木质纤维素生物质是唯一适合作为替代燃料和化学品的生物技术生产的可持续原料。然而，由于生物质的可降解性，酶转化过程是复杂的，需要简化。为此，我们开发了一种工艺，该工艺允许在单一的多物种生物膜膜反应器中将木质纤维素合并生物加工成乙醇，该反应器具有同时真菌纤维素分解酶生产和水解衍生糖的醇酵母发酵所需的需氧和厌氧条件。通过里氏木霉、酿酒酵母和树干拟杆菌的联合作用，未发酵的全浆稀酸预处理的麦草以67%的产率生产乙醇，成功地证明了这一概念的一般可行性。所取得的结果强调了该方法作为一种通用的廉价糖平台的潜力，用于通过专门编译的工业上证明强大的微生物的财团生产基于木质纤维素生物质的燃料和化学品。
Lignocellulosic biomass is uniquely suited as a sustainable feedstock for the biotechnological production of alternative fuels and chemicals. However, due to the biomass recalcitrance, the enzymatic conversion process is complex and needs to be simplified. To this end, we developed a process, which allows the consolidated bioprocessing of lignocellulose to ethanol in a single multi-species biofilm membrane reactor featuring both aerobic and anaerobic conditions necessary for the simultaneous fungal cellulolytic enzyme production and alcoholic yeast fermentation of the hydrolysis-derived sugars. The general feasibility of the concept was successfully demonstrated by producing ethanol with a 67% yield from undetoxified whole slurry dilute acid pretreated wheat straw by the combined action of Trichoderma reesei, Saccharomyces cerevisiae and Scheffersomyces stipitis. The results achieved underscore the potential of the process as a versatile cheap sugar platform for the production of fuels and chemicals based on lignocellulosic biomass by specifically compiled consortia of industrially proven robust microorganisms.