Analysis of lipidomics profile of Carya cathayensis nuts and lipid dynamic changes during embryonic development
Analysis of lipidomics profile of Carya cathayensis nuts and lipid dynamic changes during embryonic development
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DOI：
10.1016/j.foodchem.2021.130975
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发表时间：
2021-09-08
期刊：
影响因子：
8.8
通讯作者：
Huang, Jianqin
中科院分区：
文献类型：
--
作者：
Huang, Chunying;Li, Yan;Huang, Jianqin
山核桃（Carya cathayensis）坚果含有较高的脂质，具有很高的营养价值和大量的健康益处。然而，它们的脂质组成及其在胚胎发生过程中的动态变化尚未得到充分的研究。因此，采用超高效液相色谱-串联质谱（UPLCMS/MS）分析胚胎发育过程中脂质组学特征和脂质动态变化。在成熟山核桃中共鉴定出544种脂类，其中甘油脂类（59.94%）和甘油磷脂类（38.66%）比例较高。值得注意的是，二酰基甘油在胚胎发生中后期呈逐渐上升趋势，与总甘油脂和甘油磷脂相对应，表明二酰基甘油在甘油脂和甘油磷脂的积累中起着关键作用。三酰基甘油、二酰基甘油、磷脂酰乙醇胺和磷脂酰胆碱相对含量较高，不饱和脂肪酸含量丰富，特别是油酸、亚油酸和亚麻酸，主要集中在sn-2脂位。总之，我们的研究为研究山核桃脂质的营养价值提供了创新的视角。
Hickory (Carya cathayensis) nuts contain higher amount of lipids, and possess high nutritional value and substantial health benefits. However, their lipid composition and dynamic changes during embryogenesis have not been thoroughly investigated. Therefore, lipidomics profile and lipid dynamic changes during embryonic development were analyzed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLCMS/MS). Totally, 544 kinds of lipids were identified in mature hickory nuts with higher proportions of glycerolipids (59.94%) and glycerophospholipids (38.66%). Notably, diacylglycerols showed gradual uptrends, which corresponded with total glycerolipid and glycerophospholipid at middle and late stage of embryogenesis, suggesting the pivotal role of diacylglycerols in the accumulation of glycerolipids and glycerophospholipids. Moreover, triacylglycerols, diacylglycerols, phosphatidylethanolamines and phosphatidylcholines had high relative content with abundance of unsaturated fatty acids, specifically oleic acid, linoleic acid and linolenic acid, localized mainly at sn-2 lipid position. Together, our study provides innovative perspectives for studying the nutritional benefits of hickory nut lipids.