Induction of inhibitory Smad6 and Smad7 mRNA by TGF-β family members
Induction of inhibitory Smad6 and Smad7 mRNA by TGF-β family members
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DOI：
10.1006/bbrc.1998.9170
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发表时间：
1998-08-19
影响因子：
3.1
通讯作者：
ten Dijke, P
中科院分区：
文献类型：
--
作者：
Afrakhte, M;Morén, A;ten Dijke, P
Smad6和Smad7在转化生长因子- β (tgf - β)信号传导中作为细胞内拮抗剂起作用。在这里，我们报道了人类Smad6的分离，它与Smad7密切相关。Smad6和Smad7 mrna在肺癌细胞系中差异表达，可被tgf - β 1、激活素和骨形态发生蛋白-7快速直接诱导。表皮生长因子（EGF）诱导抑制SMAD mRNA的表达，证实了tgf - β与其他信号通路之间的相互作用。此外，而phobol酯PMA。单独使用对smad7mrna的表达没有影响，但它增强了tgf - β 1诱导的smad7mrna的表达。研究发现，反义Smad7 RNA的异位表达增加了tgf - β 1的作用，支持其在tgf - β信号传导中作为负调节因子的作用。因此，抑制Smads的表达是由多种刺激诱导的，包括各种tgf - β家族成员，它们的作用是拮抗的。(C) 1998学术出版社。
Smad6 and Smad7 function as intracellular antagonists in transforming growth factor-beta (TGF-beta) signaling. Here we report the isolation of human Smad6, which is closely related to Smad7. Smad6 and Smad7 mRNAs were differentially expressed in lung cancer cell lines and were rapidly and directly induced by TGF-beta 1, activin and bone morphogenetic protein-7. Cross-talk between TGF-beta and other signaling pathways was demonstrated by the finding that epidermal growth factor (EGF) induced the expression of inhibitory SMAD mRNA. Moreover, whereas the phorbol ester PMA. alone had no effect, it potentiated the TGF-beta 1-induced expression of Smad7 mRNA. Ectopic expression of anti-sense Smad7 RNA was found to increase the effect of TGF-beta 1, supporting its role as a negative regulator in TGF-beta signaling. Thus, expression of inhibitory Smads is induced by multiple stimuli, including the various TGF-beta family members, whose action they antagonize. (C) 1998 Academic Press.