3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads
3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads
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DOI：
10.1007/s10616-015-9861-1
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发表时间：
2016-08-01
期刊：
影响因子：
2.2
通讯作者：
Thurow, K.
中科院分区：
文献类型：
--
作者：
Lehmann, R.;Gallert, C.;Thurow, K.
癌症是人口中常见的问题，由年龄和有害环境影响增加引起的。因此，新的治疗策略和复合筛查是必要的。为了更好地模拟体内条件，常规的2D培养必须被三维细胞培养(3D)所取代。海藻酸盐基质三维培养是一种适合于包埋细胞形成肿瘤构筑物的方法。海藻酸盐珠的自动化制造可能是大规模制造类似癌症组织的结构的最终方法。这项研究的目的是整合用于3D细胞培养的生产、培养和筛选的全自动化系统。我们比较了自动化方法和常规的人工过程。此外，我们还研究了抗生素对这些3D细胞培养系统的影响。藻酸盐珠是通过自动和手动程序形成的。这些自动化步骤由Biomek(A(R))Cell Workstation(Celisca，Rostock，德国)进行处理。在培养的第14天和第35天手动和自动评估细胞的增殖和毒性。结果显示在孵化期间细胞聚集体的积累和扩张。然而，与第14天相比，第35天的细胞增殖和毒性略有下降。手动和自动方法的比较显示出相似的结果。因此，自动化生产完全可以取代传统的手工生产。使用自动化，3D细胞培养可以工业规模生产，并改进药物开发和筛查，以治疗癌症等严重疾病。
Cancer diseases are a common problem of the population caused by age and increased harmful environmental influences. Herein, new therapeutic strategies and compound screenings are necessary. The regular 2D cultivation has to be replaced by three dimensional cell culturing (3D) for better simulation of in vivo conditions. The 3D cultivation with alginate matrix is an appropriate method for encapsulate cells to form cancer constructs. The automated manufacturing of alginate beads might be an ultimate method for large-scaled manufacturing constructs similar to cancer tissue. The aim of this study was the integration of full automated systems for the production, cultivation and screening of 3D cell cultures. We compared the automated methods with the regular manual processes. Furthermore, we investigated the influence of antibiotics on these 3D cell culture systems. The alginate beads were formed by automated and manual procedures. The automated steps were processes by the Biomek(A (R)) Cell Workstation (celisca, Rostock, Germany). The proliferation and toxicity were manually and automatically evaluated at day 14 and 35 of cultivation. The results visualized an accumulation and expansion of cell aggregates over the period of incubation. However, the proliferation and toxicity were faintly and partly significantly decreased on day 35 compared to day 14. The comparison of the manual and automated methods displayed similar results. We conclude that the manual production process could be replaced by the automation. Using automation, 3D cell cultures can be produced in industrial scale and improve the drug development and screening to treat serious illnesses like cancer.