A novel single-labeled fluorescent oligonucleotide probe for silver(I) ion detection based on the inherent quenching ability of deoxyguanosines
A novel single-labeled fluorescent oligonucleotide probe for silver(I) ion detection based on the inherent quenching ability of deoxyguanosines
复制标题
DOI：
10.1039/c0an00669f
复制
发表时间：
2011-01-01
期刊：
影响因子：
4.2
通讯作者：
Sun, Xuping
中科院分区：
文献类型：
--
作者：
Wang, Lei;Tian, Jingqi;Sun, Xuping
基于脱氧鸟苷固有的荧光猝灭能力，设计了一种新的单标记荧光寡核苷酸（OND）探针，用于检测水溶液中的纳摩尔银离子。OND-Ag+复合物的发夹结构的形成使脱氧鸟苷接近染料，导致染料的荧光强度降低，这归因于从染料到脱氧鸟苷的光诱导电子转移。
A novel single-labeled fluorescent oligonucleotide (OND) probe for the detection of nanomolar silver(I) ion in aqueous solution is developed based on the inherent quenching ability of deoxyguanosines. The formation of a hairpin structure of the OND-Ag+ complex brings deoxyguanosines close to a dye, leading to a decreased fluorescence intensity of the dye owning to photoinduced electron transfer from the dye to deoxyguanosines.