High sensitive and multiple detection of acute myocardial infarction biomarkers based on a dual-readout immunochromatography test strip
High sensitive and multiple detection of acute myocardial infarction biomarkers based on a dual-readout immunochromatography test strip
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基于双读数免疫层析试纸条的急性心肌梗死生物标志物的高灵敏度多重检测
DOI：
10.1016/j.nano.2018.02.013
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发表时间：
2018-06-01
影响因子：
5.4
通讯作者：
Chang, Jin
中科院分区：
文献类型：
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作者：
Gong, Xiaoqun;Zhang, Bo;Chang, Jin
免疫层析试纸(ICTS)在筛选急性心肌梗死(AMI)生物标志物方面显示出较高的优势。但其灵敏度低、结果不能定量，严重限制了其临床应用。在这里，我们设计了一种基于磁性纳米颗粒(MNPs)的高灵敏度、定量和双读出的ICTS，用于检测多个急性心肌梗死生物标志物。MNPs纳米探针引起的荧光强度变化使我们能够在15min内灵敏地定量cTnI和CK-MB用于急性心肌梗死的早期诊断，相应的检测下限分别为0.049 ng/mL和0.085 ng/mL。同时，T线上的MNPs聚集物可以在2分钟内比色读出，用于急诊和重症急性心肌梗死患者的快速诊断。此外，30份临床血清样品的检测结果与电化学发光免疫分析法一致。该方法为急性心肌梗死的临床诊断和预后提供了一条新的途径。(C)2018 Elsevier Inc.保留所有权利。
Immunochromatography test strip (ICTS) displayed high advantages in screening acute myocardial infarction (AMI) biomarkers. However, the low sensitivity and nonquantitative results seriously limited its clinical application. Herein, we designed a highly sensitive, quantitative and dual-readout ICTS for assaying multiple AMI biomarkers based on magnetic nanoparticles (MNPs) quenching the fluorescence of Cy5, which was labeled on capture antibodies on test (T) lines. The changes of fluorescent intensity caused by MNPs nanoprobes enabled us to sensitively quantify cTnI and CK-MB for early diagnosis of AMI in 15 min with a corresponding detection limit of 0.049 ng/mL and 0.085 ng/mL, respectively. Meanwhile, the aggregations of MNPs on T lines allowed colorimetric readout in 2 min for rapid diagnosis of emergent and severe AMI patients. Furthermore, the detection results of 30 clinical serum samples were coincident with those by electrochemiluminescence immunoassay. So this approach is promising a new avenue for clinical diagnosis and prognosis of AMI. (C) 2018 Elsevier Inc. All rights reserved.