Expression of insulin-like growth factor 1 receptor in rat retina following optic nerve injury
Expression of insulin-like growth factor 1 receptor in rat retina following optic nerve injury
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DOI：
10.1111/aos.12096
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发表时间：
2013-09-01
影响因子：
3.4
通讯作者：
Xu, Guo-xu
中科院分区：
文献类型：
--
作者：
Bu, Shu-yang;Yu, Ge-hua;Xu, Guo-xu
目的：目的探讨视神经夹伤后视网膜神经节细胞（RGCs）凋亡及胰岛素样生长因子1受体（IGF-1 R）的变化。方法：健康Wistar大鼠70只，随机分为正常对照组和视神经损伤组。免疫组化法检测IGF-1 R的表达，流式细胞术检测RGCs凋亡。结果如下：免疫组化结果显示，视神经损伤后1小时，IGF-1 R免疫反应开始增强，24小时达高峰（P
Purpose: To investigate the apoptosis in retinal ganglion cells (RGCs) and insulin-like growth factor 1 receptor (IGF-1R) in the retina following optic nerve crush. Methods: Healthy Wistar rats (N=70) were divided into two groups: a normal control group and an optic nerve injury group. Immunohistochemistry and flow cytometry were performed to detect the expression of IGF-1R and to measure the apoptosis of RGCs, respectively. Results: Immunohistochemistry revealed that at 1hr after optic nerve injury, IGF-1R immunoreactivity began to increase and reached a maximal level at 24hr (p