Standardization of diagnostic PCR for the detection of foodborne pathogens
Standardization of diagnostic PCR for the detection of foodborne pathogens
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DOI：
10.1016/s0168-1605(02)00322-7
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发表时间：
2003-05-25
影响因子：
5.4
通讯作者：
Hoorfar, J
中科院分区：
文献类型：
--
作者：
Malorny, B;Tassios, PT;Hoorfar, J
自1980年代发现聚合酶链式反应(聚合酶链式反应)体外扩增核酸以来，它已成为分析微生物感染以及分析食品样品中微生物的一种强大的诊断工具。然而，尽管有潜力，但聚合酶链式反应既没有在常规诊断中得到广泛接受，也没有被广泛纳入标准化方法。缺乏验证和标准方案，以及试剂和设备质量参差不齐，影响了聚合酶链式反应方法从专家研究实验室向最终用户实验室的有效传播。此外，食品行业要求并期待官方批准的标准是可以理解的。认识到这一点，欧盟委员会于1999年批准了食品聚合酶链式反应(http://www.PCR.dk)，)研究项目，该项目旨在验证和标准化诊断聚合酶链式反应在检测食品中致病菌方面的使用。本文综述了聚合酶链式反应检测食源性致病菌的协调程序和标准化标准。讨论了目前诊断用聚合酶链式反应的标准化进展和发展前景。(C)2002 Elsevier Science B.V.保留所有权利。
In vitro amplification of nucleic acids using the polymerase chain reaction (PCR) has become, since its discovery in the 1980s, a powerful diagnostic tool for the analysis of microbial infections as well as for the analysis of microorganisms in food samples. However, despite its potential, PCR has neither gained wide acceptance in routine diagnostics nor been widely incorporated in standardized methods. Lack of validation and standard protocols, as well as variable quality of reagents and equipment, influence the efficient dissemination of PCR methodology from expert research laboratories to end-user laboratories. Moreover, the food industry understandably requires and expects officially approved standards. Recognizing this, in 1999, the European Commission approved the research project, FOOD-PCR (http://www.PCR.dk), which aims to validate and standardize the use of diagnostic PCR for the detection of pathogenic bacteria in foods. The present review focuses on the harmonization procedure and standardization criteria for detection of foodborne pathogens by PCR. The progress of standardization so far and future perspectives of diagnostic PCR are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.