Luteal macrophage conditioned medium affects steroidogenesis in porcine granulosa cells
Luteal macrophage conditioned medium affects steroidogenesis in porcine granulosa cells
复制标题
DOI：
10.1016/s1642-431x(12)60049-7
复制
发表时间：
2011-07-01
影响因子：
2.1
通讯作者：
Slomczynska, Maria
中科院分区：
文献类型：
--
作者：
Duda, Malgorzata;Knet, Malgorzata;Slomczynska, Maria
本研究的目的是研究黄体巨噬细胞条件培养基（LMCM）对培养的颗粒细胞产生孕酮和雌二醇的影响。在不含或不含LMCM的条件下，分别在100 ng/ml LH、FSH或催乳素的条件下，培养猪颗粒细胞48 h。用放射免疫法测定孕酮和雌二醇浓度。用组织化学和免疫细胞化学分别检测颗粒细胞中δ 5,3 β -羟基类固醇脱氢酶（3 β - hsd）的活性和存在。LMCM刺激基础和LH-， FSH-或催乳素诱导的黄体酮分泌。同样，LMCM增强了被测细胞中3 β - hsd的基础和刺激活性。相比之下，LMCM降低了LH和催乳素诱导的雌二醇分泌，但增加了fsh诱导的雌二醇分泌。这些数据证明了LMCM对颗粒孕酮产生的明显刺激作用。综上所述，巨噬细胞分泌的物质通过3 β - hsd活性以旁分泌方式调节促性腺激素对卵泡孕酮分泌的影响。生殖生物学，2011(2):117-134。
The purpose of the study was to examine the effect of luteal macrophage conditioned medium (LMCM) on progesterone and estradiol production by cultured granulosa cells. Porcine granulosa cells were cultured for 48 h with or without LMCM in the absence or presence of 100 ng/ml LH, FSH or prolactin. Progesterone and estradiol concentrations were measured by radioimmunoassay. Granulosa cells were analyzed histochemically and immunocytochemically for the activity and presence of Delta 5, 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), respectively. LMCM stimulated basal and LH-, FSH- or prolactin-induced progesterone secretion. Similarly, LMCM augmented basal and stimulated activity of 3 beta-HSD in the examined cells. In contrast, LMCM decreased LH- and prolactin-induced estradiol secretion but increased FSH-induced estradiol secretion. These data demonstrate the clear stimulatory effect of LMCM on granulosal progesterone production. It is concluded that substances secreted by macrophages modulate gonadotropin effect on follicular progesterone secretion in a paracrine manner via 3 beta-HSD activity. Reproductive Biology 2011 2: 117-134.