Glucose transporter/T1R3-expressing cells in rat tracheal epithelium
Glucose transporter/T1R3-expressing cells in rat tracheal epithelium
复制标题
DOI：
10.1111/j.1469-7580.2012.01522.x
复制
发表时间：
2012-08-01
影响因子：
2.4
通讯作者：
Sbarbati, Andrea
中科院分区：
文献类型：
--
作者：
Merigo, Flavia;Benati, Donatella;Sbarbati, Andrea
葡萄糖转运在维持气道表面液体（ASL）中的低糖浓度中起着重要作用，这对于粘膜纤毛清除和细菌定植至关重要。实验证据表明，肺/气道细胞中的葡萄糖/己糖摄取通过两种结构不同的葡萄糖转运蛋白途径发生：Na+依赖性葡萄糖转运蛋白（SGLT家族）和促进性葡萄糖转运蛋白（GLUT家族）。在这项研究中，我们研究了肠道的主要葡萄糖转运蛋白，GLUT 2，GLUT 5，SGLT 1和T1 R3味觉受体亚基，在大鼠气管的表达，用免疫组织化学和免疫电镜，并比较它们使用双标记共聚焦显微镜。我们发现GLUT 2、GLUT 5、SGLT 1和T1 R3在不同的细胞类型中选择性表达。T1 R3和GLUT 2主要在孤立化学感受器细胞（SCC）和纤毛细胞亚群中表达，GLUT 5存在于SCC亚群和分泌细胞中，SGLT 1仅在独特的细胞类型SCC中表达。此外，我们证明了T1 R3在SCC中与SGLT 1共定位，在纤毛细胞中与GLUT 2转运蛋白共定位。总之，这些研究结果表明，不同的细胞类型与ASL中葡萄糖的摄取相关，并且由于它们的T1 R3表达，SCC和纤毛细胞最有可能参与ASL中的化学感受过程。
Glucose transport plays an important role in maintaining low sugar concentration in airway surface liquid (ASL), which is critical for mucociliary clearance and bacterial colonization. Experimental evidence indicates that glucose/hexose uptake in lung/airway cells occurs by means of two structurally distinct glucose transporter pathways: the Na+-dependent glucose transporters (SGLT family) and the facilitative glucose transporters (GLUT family). In this study, we examined the expression of the major glucose transporters of the intestine, GLUT2, GLUT5, SGLT1 and T1R3 taste receptor subunit, in the trachea of rats using immunohistochemistry and immunoelectron microscopy, and compared them using double-labeled confocal microscopy. We found that GLUT2, GLUT5, SGLT1 and T1R3 are selectively expressed in different cell types. T1R3 and GLUT2 are predominantly expressed in subsets of solitary chemoreceptor cells (SCCs) and ciliated cells, GLUT5 is present in subsets of SCCs and in secretory cells, and SGLT1 is exclusively expressed in a unique cell type, SCCs. Furthermore, we demonstrated that T1R3 is colocalized with SGLT1 in SCCs and with GLUT2 transporter in ciliated cells. In conclusion, these findings reveal that different cell types are associated with the uptake of glucose in ASL and that, due to their T1R3 expression, SCCs and ciliated cells are most likely to participate in the chemosensory process in ASL.