Molecular analysis of HIV strains from a cluster of worker infections in the adult film industry, Los Angeles 2004
Molecular analysis of HIV strains from a cluster of worker infections in the adult film industry, Los Angeles 2004
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DOI：
10.1097/01.aids.0000218558.82402.59
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发表时间：
2006-04-04
期刊：
影响因子：
3.8
通讯作者：
Kalish, ML
中科院分区：
文献类型：
--
作者：
Brooks, JT;Robbins, KE;Kalish, ML
目的：2004年4月，13名易感染艾滋病毒的妇女在受雇为制作成人电影而进行各种性行为时接触了一名严重感染艾滋病毒1的男子;随后发现3名妇女感染了艾滋病毒（发病率为23%）。作为这一感染集群的调查的一部分，我们评估是否从感染的individuals.Methods收集的病毒株显着related：我们确定了核苷酸序列从C2 V3 C3和gp 41区域的env和p17区域的gag病毒从三个感染的个体，从他们的标本。然后，我们将这些序列与来自可用参考菌株的可比序列进行遗传学比较。基因型和表型抗逆转录病毒药物耐药性测定血浆病毒从男性指数的情况下，一个女性接触在一个单独的商业laborator.Results：从男性指数的情况下，两个感染的妇女的HIV毒株的env和gag序列是100%相似。男性索引病例的病毒基因分型确定了12个突变，这些突变代表了B亚型共有序列中已知的自然发生的多态性，与抗逆转录病毒药物耐药性无关。对来自女性接触者的病毒进行基因分型，发现了10个突变，所有这些突变都与来自男性索引病例的病毒相同。表型分析表明，这两种病毒是敏感的所有抗逆转录病毒药物tested.Conclusion：分子和病毒学的数据强烈支持流行病学的结论，这些妇女感染了同一株艾滋病毒通过职业暴露于一个人与急性艾滋病毒感染。(C)2006年利平科特威廉姆斯&威尔金斯。
Objectives: In April 2004, 13 susceptible women were exposed to a single acutely HIV-1-infected man while employed to perform various sex acts for the production of adult films; three women were subsequently found to have acquired HIV infection (23% attack rate). As part of the investigation of this infection cluster, we evaluated whether viral strains collected from infected individuals were significantly related.Methods: We determined nucleotide sequences from the C2V3C3 and gp41 region of env and the p17 region of gag in viruses from the three infected individuals from whom specimens were available. We then compared these sequences phylogenetically to comparable sequences from available reference strains. Genotypic and phenotypic antiretroviral drug resistance was determined for plasma virus from the male index case and one female contact at a separate commercial laboratory.Results: The env and gag sequences of the HIV strains from the male index case and two of the infected women were 100% similar. Genotyping of the male index case's virus identified 12 mutations, which represented known naturally occurring polymorphisms in the subtype B consensus sequence that are not associated with antiretroviral drug resistance. Genotyping of the virus from the female contact identified 10 mutations, all of which were shared by the virus from the male index case. Phenotyping demonstrated that both viruses were susceptible to all antiretroviral drugs tested.Conclusion: Molecular and virological data strongly support the epidemiological conclusion that these women were infected with an identical strain of HIV through occupational exposure to an individual with an acute HIV infection. (C) 2006 Lippincott Williams & Wilkins.