Front-End Electron Transfer Dissociation: A New Ionization Source
Front-End Electron Transfer Dissociation: A New Ionization Source
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DOI：
10.1021/ac401783f
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发表时间：
2013-09-03
影响因子：
7.4
通讯作者：
Compton, Philip D.
中科院分区：
文献类型：
--
作者：
Earley, Lee;Anderson, Lissa C.;Compton, Philip D.
电子转移解离 (ETD) 是一种提供高效裂解，同时将少量能量沉积到振动模式中的技术，已广泛集成到蛋白质组学工作流程中。目前该技术的实现以及质子转移等其他离子-离子反应都涉及复杂的硬件，缺乏鲁棒性，并且对其所连接的仪器施加了严格的设计限制。本文描述的是一种新颖的、基于放电的试剂离子源，其位于质谱仪的第一差动泵浦区域中。发现试剂源可在较长时间内产生强烈的试剂离子信号，同时对前体离子信号没有可测量的影响。此外，该源构建起来很简单，并且可以在任何仪器上实施 ETD，而无需修改封装。最后，在混合质谱仪的背景下，将试剂离子源重新定位到质谱仪的前端，使得完整蛋白质的气相询问成为可能。
Electron transfer dissociation (ETD), a technique that provides efficient fragmentation while depositing little energy into vibrational modes, has been widely integrated into proteomics workflows. Current implementations of this technique, as well as other ion-ion reactions like proton transfer, involve sophisticated hardware, lack robustness, and place severe design limitations on the instruments to which they are attached. Described herein is a novel, electrical discharge-based reagent ion source that is located in the first differentially pumped region of the mass spectrometer. The reagent source was found to produce intense reagent ion signals over extended periods of time while having no measurable impact on precursor ion signal. Further, the source is simple to construct and enables implementation of ETD on any instrument without modification to footprint. Finally, in the context of hybrid mass spectrometers, relocation of the reagent ion source to the front of the mass spectrometer enables new approaches to gas phase interrogation of intact proteins.