Simultaneous determination of twelve biogenic amines in human urine as potential biomarkers of inflammatory bowel diseases by capillary electrophoresis - tandem mass spectrometry
Simultaneous determination of twelve biogenic amines in human urine as potential biomarkers of inflammatory bowel diseases by capillary electrophoresis - tandem mass spectrometry
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DOI：
10.1016/j.jpba.2020.113294
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发表时间：
2020-07-15
影响因子：
3.4
通讯作者：
Mikus, Peter
中科院分区：
文献类型：
--
作者：
Marakova, Katarina;Piest'ansky, Juraj;Mikus, Peter
生物胺（BA）是一类广泛的生物活性物质，其在人体内的存在可以为许多不同的病理学（包括慢性炎症）提供重要的诊断信息。在这项工作中，开发了毛细管电泳（CE）与串联质谱（MS/MS）联用的方法，用于同时测定人尿液中12种BA（组胺、血清素、多巴胺、去甲肾上腺素、肾上腺素、腐胺、尸胺、精胺、亚精胺、酪胺、色胺、苯乙胺）作为炎症性肠病（IBD）的潜在生物标志物。电泳分离在未涂覆的熔融石英毛细管（内径 50 μm）中进行，使用 50 mM 甲酸（pH 2.0）作为背景电解质。分析物的可靠鉴定基于 CE 中的时间分辨率和三重四极杆 MS/MS 中的质量分辨率的组合。所提出的 CE-MS/MS 方法的总分析时间不到 10 分钟，检测限在 4.47-144 ng/mL 范围内。日内和日间精度范围分别为89.75-109.4%和89.99-110.2%，日内和日间精度的RSD值分别低于14%和13%。三个浓度水平下样品的回收率范围为 81.73-105.6%，精度不超过 9.9%。 CE-MS/MS 方法的良好性能参数凸显了其在常规临床应用中的有用性。在这项工作中，CE-MS/MS 方法首次应用于临床人体样本中 BA 的分析。结果显示，与对照组相比，IBD 患者研究组的血清素和去甲肾上腺素显着降低，组胺和亚精胺显着增加。这些发现可用于研究和阐明IBD或相关治疗的机制。 (C) 2020 Elsevier B.V. 保留所有权利。
Biogenic amines (BA) are a broad group of biologically active substances, the presence of which in the human body can provide important diagnostic information for many various pathologies, including chronic inflammation. In this work, a capillary electrophoresis (CE) hyphenated with tandem mass spectrometry (MS/MS) method was developed for the simultaneous determination of twelve BA (histamine, serotonin, dopamine, norepinephrine, epinephrine, putrescine, cadaverine, spermine, spermidine, tyramine, tryptamine, phenylethylamine) in human urine as potential biomarkers of inflammatory bowel diseases (IBD). The electrophoretic separations were carried out in an uncoated fused silica capillary (I.D. 50 mu m) using 50 mM formic acid (pH 2.0) as a background electrolyte. A reliable identification of the analytes was based on the combination of time resolution in CE and mass resolution in triple quadrupole MS/MS. The total analysis time of the proposed CE-MS/MS method was less than 10 min with the limits of detection in the range of 4.47-144 ng/mL. The intra- and inter-day accuracy ranged in the intervals 89.75-109.4% and 89.99-110.2%, respectively, with the RSD values for the intra- and inter-day precision lower than 14 and 13 %, respectively. The recovery values for the samples spiked at three concentration levels ranged from 81.73-105.6% with a precision not exceeding 9.9 %. The favorable performance parameters of the CE-MS/MS method highlighted its usefulness for routine clinical applications. In this work, the CE-MS/MS method was applied, for the first time, to the analytical profiling of the BA in clinical human samples. The obtained results showed a statistically significant decrease of serotonin and norepinephrine, and an increase of histamine and spermidine, in the studied group of IBD patients when compared with the control group. These findings could be utilized in studying and clarifying the mechanisms of IBD or relevant therapy. (C) 2020 Elsevier B.V. All rights reserved.