Alternative Splicing Plays a Critical Role in Maintaining Mineral Nutrient Homeostasis in Rice (Oryza sativa)
Alternative Splicing Plays a Critical Role in Maintaining Mineral Nutrient Homeostasis in Rice (Oryza sativa)
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DOI：
10.1105/tpc.18.00051
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发表时间：
2018-10-01
期刊：
影响因子：
11.6
通讯作者：
Zheng, Luqing
中科院分区：
文献类型：
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作者：
Dong, Chunlan;He, Fei;Zheng, Luqing
前 mRNA 的选择性剪接 (AS) 可促进转录组和蛋白质组的多样性，并在广泛的生物过程中发挥重要作用。然而，AS 在维持植物矿质养分稳态中的作用尚不清楚。为了阐明这一作用，我们获得了在存在或不存在几种矿物质营养素（铁、锌、铜、锰和磷）的情况下生长的水稻 (Oryza sativa) 根的全转录组 RNA 测序数据。我们的系统分析揭示了 13,291 个选择性剪接基因，相当于水稻基因组中多外显子基因的 53.3%。由于差异表达基因和差异选择性剪接基因之间的重叠很小，因此仅通过分析差异表达基因来限制对植物对矿物质缺乏的反应的分子理解。我们发现 AS 的目标具有高度的营养特异性。为了验证 AS 在矿物质营养中的作用，我们对编码在 AS 中发挥作用的 Ser/Arg (SR) 蛋白的基因突变体进行了表征。我们确定了几种 SR 蛋白作为 Zn、Mn 和 P 营养的关键调节因子，并表明三种 SR 蛋白编码基因调节水稻叶和芽之间的 P 吸收和再动员，表明 AS 在调节水稻矿质营养稳态中具有关键作用。
Alternative splicing (AS) of pre-mRNAs promotes transcriptome and proteome diversity and plays important roles in a wide range of biological processes. However, the role of AS in maintaining mineral nutrient homeostasis in plants is largely unknown. To clarify this role, we obtained whole transcriptome RNA sequencing data from rice (Oryza sativa) roots grown in the presence or absence of several mineral nutrients (Fe, Zn, Cu, Mn, and P). Our systematic analysis revealed 13,291 alternatively spliced genes, representing similar to 53.3% of the multiexon genes in the rice genome. As the overlap between differentially expressed genes and differentially alternatively spliced genes is small, a molecular understanding of the plant's response to mineral deficiency is limited by analyzing differentially expressed genes alone. We found that the targets of AS are highly nutrient-specific. To verify the role of AS in mineral nutrition, we characterized mutants in genes encoding Ser/Arg (SR) proteins that function in AS. We identified several SR proteins as critical regulators of Zn, Mn, and P nutrition and showed that three SR protein-encoding genes regulate P uptake and remobilization between leaves and shoots of rice, demonstrating that AS has a key role in regulating mineral nutrient homeostasis in rice.