Immune-related gene expression in response to H11N9 low pathogenic avian influenza virus infection in chicken and Pekin duck peripheral blood mononuclear cells
Immune-related gene expression in response to H11N9 low pathogenic avian influenza virus infection in chicken and Pekin duck peripheral blood mononuclear cells
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DOI：
10.1016/j.molimm.2009.01.025
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发表时间：
2009-05-01
影响因子：
3.6
通讯作者：
Cardona, Carol J.
中科院分区：
文献类型：
--
作者：
Adams, Sean C.;Xing, Zheng;Cardona, Carol J.
鸭和鸡是禽流感病毒（AIV）的重要宿主，对AIV的感染反应各不相同。通常，鸭的AIV感染是无症状的和持久的，与在鸡中观察到的临床上明显的和短暂的感染相反。这些差异可能部分是由于宿主对AN感染的反应。采用实时荧光定量PCR技术，检测了低致病性禽流感病毒H11 N9感染后鸡和北京鸭外周血单个核细胞（PBMC）中免疫相关基因的表达。鸡PBMC表达IL-1 β和IL-6的水平与哺乳动物相似，而鸭PBMC表达水平最低或无变化。同样，鸭IFN-β的表达几乎不受影响，而鸡的表达高度上调。鸡IFN-γ的表达水平高于鸭IFN-γ，而IFN-α在两个物种中的表达相似。IL-2在鸭PBMC中在感染早期升高，但在实验结束时恢复到基线水平;相反，IL-2在鸡PBMC中在晚期时间点被弱诱导。TLR-7和MHC I类分子表达在物种之间是保守的，而鸭MHC II类表达下调，鸡的表达没有变化。这些结果显示了不同物种之间促炎细胞因子和IFN的不同PBMC表达模式。促炎细胞因子和IFN表达的差异反映了在鸭中观察到的无症状和持久感染以及在鸡中观察到的临床体征和快速清除的趋势。这些结果突出了两个物种的宿主对AIV的反应的重要差异，这两个物种被认为是AIV流行株的发生和维持的关键。(C)2009爱思唯尔有限公司版权所有。
The duck and chicken are important hosts of avian influenza virus (AIV) with distinctive responses to infection. Frequently, AIV infections in ducks are asymptomatic and long-lasting in contrast to the clinically apparent and transient infections observed in chickens. These differences may be due in part to the host response to AN infection. Using real-time quantitative PCR, we examined the expression of immune-related genes in response to low pathogenic AIV H11N9 infection in peripheral blood mononuclear cells (PBMC) isolated from the blood of chickens and Pekin ducks. While chicken PBMC expressed IL-1 beta and IL-6 at high levels similar to mammalian species, duck PBMC expression levels were minimal or unchanged. Similarly, duck IFN-beta expression was nearly unaffected, whereas chicken expression was highly upregulated. Chicken IFN-gamma was expressed to higher levels than duck IFN-gamma, while IFN-alpha was expressed similarly by both species. IL-2 was elevated early in infection in duck PBMC, but returned to baseline levels by the end of the experiment; in contrast, IL-2 was weakly induced in chicken PBMC at late time points. TLR-7 and MHC class I molecule expressions were conserved between species, whereas duck MHC class II expression was downregulated and chicken expression was unchanged. These results show distinct PBMC expression patterns of pro-inflammatory cytokines and IFNs between species. The differences in pro-inflammatory cytokine and IFN expression reflect the asymptomatic and lasting infection observed in ducks and the tendency towards clinical signs and rapid clearance seen in chickens. These results highlight important differences in the host response to AIV of two species thought to be critical in the genesis and maintenance of epidemic strains of AIV. (C) 2009 Elsevier Ltd. All rights reserved.