Biphasic kinetics of a methanotrophic community is a combination of growth and increased activity per cell
Biphasic kinetics of a methanotrophic community is a combination of growth and increased activity per cell
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DOI：
10.1111/j.1574-6941.2009.00782.x
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发表时间：
2010-01-01
影响因子：
4.2
通讯作者：
Bodelier, Paul L. E.
中科院分区：
文献类型：
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作者：
Steenbergh, Anne K.;Meima, Marion M.;Bodelier, Paul L. E.
由于甲烷氧化细菌（MOB）是温室气体甲烷的唯一生物汇，因此需要了解这些细菌在各种生态系统中的功能，以了解在全球甲烷排放中观察到的动态。MOB的活性通常通过甲烷氧化试验来评估。由此产生的甲烷消耗曲线通常遵循初始和诱导甲烷氧化活性的双相模式，通常被解释为分别代表原位活性和总MOB群落。在甲烷耗竭曲线过渡点前、点处和点后分别进行土壤孵育，定量pcr的应用表明，在初始阶段，pmoA-mRNA已经产生，细胞也开始大量生长。此外，Ia型和II型MOB表现出明显不同的行为，这可以解释为不同的生态策略。为了正确解释甲烷氧化测定，建议使用小时间窗来计算甲烷氧化活性，以避免大量细胞生长。
Because methane-oxidizing bacteria (MOB) are the only biological sink for the greenhouse gas methane, knowledge of the functioning of these bacteria in various ecosystems is needed to understand the dynamics observed in global methane emission. The activity of MOB is commonly assessed by methane oxidation assays. The resulting methane depletion curves often follow a biphasic pattern of initial and induced methane oxidation activity, often interpreted as representing the in situ active and total MOB community, respectively. The application of quantitative-PCR on soil incubations, which were stopped before, at and after the transition point in the methane-depletion curve, demonstrated that both pmoA-mRNA was produced as well as substantial cell growth took place already in the initial phase. In addition, type Ia and II MOB displayed markedly different behaviour, which can be interpreted as ecologically different strategies. For the correct interpretation of methane oxidation assays, the use of small time windows is recommended to calculate methane oxidation activities to avoid substantial cell growth.