Prostate-specific antigen in vaginal fluid as a biologic marker of condom failure
Prostate-specific antigen in vaginal fluid as a biologic marker of condom failure
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DOI：
10.1016/s0010-7824(99)00013-x
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发表时间：
1999-03-01
期刊：
影响因子：
2.9
通讯作者：
Hortin, G
中科院分区：
文献类型：
--
作者：
Macaluso, M;Lawson, L;Hortin, G
40名妇女参加了三次门诊，期间她们接触到了伴侣的精液(L 10亩，L 100亩，精液1毫升)。在每次就诊时，他们在接触伴侣的精液之前、接触后立即、以及接触后1、24和48小时采集阴道液体样本。用双抗体夹心法测定PSA。暴露前拭子的平均PSA水平在0.43至0.88 ng/mL之间。接触10亩L、100亩L和1毫升精液后，平均PSA值分别为193、472和19,098，1小时内下降，48小时恢复本底水平，证实本方法是检测近期精液暴露的一种敏感而特异的方法，并提示PSA水平与暴露强度和暴露后时间有关。该方法在避孕套功效研究中的应用提供了避孕套失效的客观证据，增强了对自我报告的理解。(C)1999 Elsevier Science Inc.保留所有权利。
Forty women participated in three clinic visits during which they were exposed to their partner's semen (10 mu L, 100 mu L, and 1 ml). At each visit they took vaginal fluid samples before exposure to their partner's semen, immediately after, and at 1, 24, and 48 h after exposure. PSA was measured with an enzyme-linked immunoassay. The mean PSA level for preexposure swabs ranged between 0.43 and 0.88 ng/mL. The mean PSA levels were 193 immediately after exposure to 10 mu L, 472 after 100 mu L, and 19,098 after 1 ml. The PSA levels declined within 1 h, and returned to background at 48 h. The findings confirm that our procedure is a sensitive and specific method for detecting recent semen exposure, and indicate that PSA levels depend on exposure intensity and time since exposure. Application of this method in condom efficacy studies provides objective evidence of condom failure that enhances the interpretation of self-report. (C) 1999 Elsevier Science Inc. All rights reserved.