Ultrasound-based Pulse Wave Velocity Evaluation in Mice
Ultrasound-based Pulse Wave Velocity Evaluation in Mice
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DOI：
10.3791/54362
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发表时间：
2017-02-01
影响因子：
1.2
通讯作者：
Faita, Francesco
中科院分区：
文献类型：
--
作者：
Di Lascio, Nicole;Kusmic, Claudia;Faita, Francesco
可以通过计算脉搏波速度（PWV）来评估动脉硬度，即，脉搏波在导管中传播的速度。该参数在小型啮齿动物模型中的研究越来越多，在该模型中，该参数用于评估与特定基因型/治疗相关的血管功能改变或用于表征心血管疾病进展。该方案描述了一种图像处理算法，该算法仅使用超声（US）图像在小鼠中进行无创动脉PWV测量。所提出的技术已被用来评估腹主动脉PWV在小鼠和评估其年龄相关的changes.Abdominal主动脉US扫描获得小鼠在气体麻醉下使用一个特定的US设备配备了高频US探头。分析B模式和脉冲波多普勒（PW-多普勒）图像，以分别获得直径和平均速度瞬时值。为此，采用边缘检测和轮廓跟踪技术。单拍平均直径和速度波形在时间上对齐并组合，以实现直径-速度（InD-V）环。PWV值从环路的线性部分的斜率获得，其对应于收缩早期phase.With本方法，关于小鼠腹主动脉的解剖和功能信息可以非侵入性地实现。只需要处理的US图像，它可能是一个有用的工具，在小鼠的不同动脉部位的弹性特性方面的非侵入性表征。本技术的应用可以容易地扩展到其他血管区域，例如颈动脉，从而提供了获得多部位动脉硬度评估的可能性。
Arterial stiffness can be evaluated by calculating pulse wave velocity (PWV), i.e., the speed with which the pulse wave travels in a conduit vessel. This parameter is being increasingly investigated in small rodent models in which it is used for assessing alterations in vascular function related to particular genotypes/treatments or for characterizing cardiovascular disease progression. This protocol describes an image processing algorithm which leads to non-invasive arterial PWV measurement in mice using ultrasound (US) images only. The proposed technique has been used to assess abdominal aorta PWV in mice and evaluate its age-associated changes.Abdominal aorta US scans are obtained from mice under gaseous anesthesia using a specific US device equipped with high-frequency US probes. B-mode and Pulse-Wave Doppler (PW-Doppler) images are analyzed in order to obtain diameter and mean velocity instantaneous values, respectively. For this purpose, edge detection and contour tracking techniques are employed. The single-beat mean diameter and velocity waveforms are time aligned and combined in order to achieve the diameter-velocity (InD-V) loop. PWV values are obtained from the slope of the linear part of the loop, which corresponds to the early systolic phase.With the present approach, anatomical and functional information about the mouse abdominal aorta can be non-invasively achieved. Requiring the processing of US images only, it may represent a useful tool for the non-invasive characterization of different arterial sites in the mouse in terms of elastic properties. The application of the present technique can be easily extended to other vascular districts, such as the carotid artery, thus providing the possibility to obtain a multi-site arterial stiffness assessment.