Molecular epidemiology of Paenibacillus larvae larvae and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
Molecular epidemiology of Paenibacillus larvae larvae and incidence of American foulbrood in Argentinean honeys from Buenos Aires province
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DOI：
10.1080/00218839.2004.11101124
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发表时间：
2004-01-01
影响因子：
1.9
通讯作者：
Aguilar, OM
中科院分区：
文献类型：
--
作者：
Alippi, AM;Reynaldi, FJ;Aguilar, OM
幼虫类芽孢杆菌幼虫是美洲蜜蜂幼虫腐臭病的病原体，在世界各地发生，在阿根廷的所有养蜂区都有发现。对从布宜诺斯艾利斯省（阿根廷）蜂箱中采集的394份蜂蜜样品进行微生物分析，从三年（1999-2001年）的采样中得出219例阳性病例（55.6%）。P l. 1999年、2000年和2001年3个年份的幼虫感染率分别为68.1%（n = 160）、47.1%（n = 102）和46.2%（n = 132）。三年研究的孢子污染平均值显示持续下降，这可能是由于养蜂人通过饲养蜜蜂进行卫生行为和减少抗生素治疗来控制AFB的良好疾病管理做法。P l.从蜂蜜中分离的幼虫群体基于DNA指纹，使用基于重复序列的聚合酶链反应技术（rep-PCR），用BOX-和REP-序列特异性引物进行表征。分离物之间存在4种不同的模式，命名为A、B、C和D。在以前的研究中没有观察到基因型D;这一发现可能与自1997年以来阿根廷新引进的疾病有关，当时只有三种基因型（A，B和C）得到证实。将所获得的rep-PCR指纹图谱与由世界范围的Pl.幼虫品系在来自世界18个不同国家的菌株集合中发现了相同的4种基因型模式。需要指出的是，模式C仅在阿根廷和位于边境线的乌拉圭样本中发现，这表明基因型C可能来自基因型A，并从阿根廷传播到乌拉圭。这些研究结果支持了这一假设，即美国foulbrood疾病暴露于有限的选择压力，从气候和环境来源。
Paenibacillus larvae larvae, the causative agent of American foulbrood disease of honey bee larvae occurs throughout the world and is found in all beekeeping areas of Argentina. Microbiological analysis of 394 honey samples obtained from bee hives from Buenos Aires province (Argentina) from three years of sampling (1999-2001) yielded 219 positive cases (55.6%). The incidence of P l. larvae infected honey samples for 1999 was 68.1% (n = 160), for 2000 47.1% (n = 102), and 46.2% for 2001 (n = 132). The mean values of spore contamination for the three-year study showed a continuous reduction, probably due to good practices of disease management by beekeepers by breeding bees for hygienic behaviour and reduction of antibiotic treatments for control of AFB. P l. larvae populations isolated from honey were characterized on the basis of DNA fingerprints using the repetitive-sequence-based polymerase chain reaction technique (rep-PCR) with BOX- and REP- sequence- specific primers. Four distinctive patterns, named A, B, C, and D, were distinguishable among the isolates. Genotype D was not observed in previous studies; this finding could be correlated with a new introduction of the disease in Argentina since 1997 when only three genotypes (A, B, and C) were confirmed. The rep-PCR fingerprint patterns obtained were compared with the patterns generated by a world-wide collection of P l. larvae strains. The same 4 genotypes patterns were found within a collection of strains from 18 different countries of the world. It is important to point out that pattern C was only found in Argentina and in one sample from Uruguay located in the border line, suggesting that genotype C could have been derived from genotype A and disseminated to Uruguay from Argentina. These findings support the hypothesis that American foulbrood disease is exposed to a limited selective pressure from climatic and environmental sources.