Blood clearance and tissue distribution of PEGylated and non-PEGylated gold nanorods after intravenous administration in rats
Blood clearance and tissue distribution of PEGylated and non-PEGylated gold nanorods after intravenous administration in rats
复制标题
DOI：
10.2217/nnm.10.122
复制
发表时间：
2011-02-01
期刊：
影响因子：
5.5
通讯作者：
Manohar, Srirang
中科院分区：
文献类型：
--
作者：
Lankveld, Danielle P. K.;Rayavarapu, Raja G.;Manohar, Srirang
目标：开发并确定金纳米棒的安全性，其长宽比可以调整以获得 650 至 850 nm 之间的等离激元峰值，作为诊断和治疗应用的对比增强剂。材料与方法：在这项研究中，我们比较了十六烷基三甲基溴化铵（CTAB）封端和聚乙二醇（PEG）包被的金纳米棒在大鼠尾静脉静脉注射后的血液清除率和组织分布。采用电感耦合等离子体质谱法定量测定血液和各器官中的金含量。结果与讨论：CTAB 覆盖的金纳米棒几乎立即（< 15 分钟）从血液循环中清除，而金纳米棒的聚乙二醇化则导致血液循环延长，半衰期为 19 小时，并且组织分布更广泛。对于 CTAB 覆盖的金纳米棒，组织分布仅限于肝脏、脾脏和肺，而聚乙二醇化的金纳米棒还分布到肾脏、心脏、胸腺、大脑和睾丸。金纳米棒的聚乙二醇化导致脾脏成为每克器官暴露量最高的器官，而对于非聚乙二醇化 CTAB 封端的金纳米棒，肝脏是每克器官暴露量最高的器官。结论：金纳米棒的聚乙二醇化导致了血液清除率的延长和脾脏中最高的器官暴露。鉴于观察到的血液循环中存在的时间范围（长达 48 小时），聚乙二醇化金纳米棒可以被认为是用于治疗和诊断成像目的的有希望的候选者。
Aims: To develop and determine the safety of gold nanorods, whose aspect ratios can be tuned to obtain plasmon peaks between 650 and 850 nm, as contrast enhancing agents for diagnostic and therapeutic applications. Materials & methods: In this study we compared the blood clearance and tissue distribution of cetyl trimethyl ammonium bromide (CTAB)-capped and polyethylene glycol (PEG)-coated gold nanorods after intravenous injection in the tail vein of rats. The gold content in blood and various organs was measured quantitatively with inductively coupled plasma mass spectrometry. Results & discussion: The CTAB-capped gold nanorods were almost immediately (< 15 min) cleared from the blood circulation whereas the PEGylation of gold nanorods resulted in a prolonged blood circulation with a half-life time of 19 h and more wide spread tissue distribution. While for the CTAB-capped gold nanorods the tissue distribution was limited to liver, spleen and lung, the PEGylated gold nanorods also distributed to kidney, heart, thymus, brain and testes. PEGylation of the gold nanorods resulted in the spleen being the organ with the highest exposure, whereas for the non-PEGylated CTAB-capped gold nanorods the liver was the organ with the highest exposure, per gram of organ. Conclusion: The PEGylation of gold nanorods resulted in a prolongation of the blood clearance and the highest organ exposure in the spleen. In view of the time frame (up to 48 h) of the observed presence in blood circulation, PEGylated gold nanorods can be considered to be promising candidates for therapeutic and diagnostic imaging purposes.