Monoclonal antibodies defining blood group A variants with difucosyl type 1 chain (ALeb) and difucosyl type 2 chain (ALey).
Monoclonal antibodies defining blood group A variants with difucosyl type 1 chain (ALeb) and difucosyl type 2 chain (ALey).
复制标题
使用二岩藻糖基 1 型链 (ALeb) 和二岩藻糖基 2 型链 (ALey) 定义 A 型血型变体的单克隆抗体。
DOI：
10.1021/bi00343a024
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Hakomori,S
中科院分区：
文献类型：
--
作者：
Clausen,H;McKibbin,JM;Hakomori,S
生物化学肿瘤学/膜研究项目，Fred哈钦森癌症研究中心，病理学、微生物学和免疫学系，华盛顿大学，西雅图，华盛顿98104，以及生物化学系，亚拉巴马大学，伯明翰，伯明翰，亚拉巴马35294，1985年4月12日接收摘要：已经建立了三种分泌单克隆抗体HH 1、HH 2和HH 3的杂交瘤，定义了不同的二岩藻糖基A结构（ALEb或ALey）。抗体HH 1（IgG 2a）与二岩藻糖基A结构特异性反应，而与1型或2型链无关，而抗体HH 2（IgG 3）仅与二岩藻糖基2型链A（ALey）反应，不与二岩藻糖基1型链或单岩藻糖基2型链反应。抗体HH 3（IgG 2a）仅与1型二岩藻糖基链A（ALEb）反应，不与1型单岩藻糖基链A或2型单岩藻糖基和二岩藻糖基链A反应。这些杂交瘤抗体通过用纯化的糖脂抗原免疫小鼠获得，并通过它们与特定糖脂结构的反应性进行选择。这些抗体与先前建立的单克隆抗体AH-21（对1型单岩藻糖基链A具有特异性）和单克隆抗体TH-1（对3型链A具有特异性）一起，对于确定细胞和组织中存在的血型A变体非常有用。血型A决定簇是一种公认的三糖，GalN Aca 1-3 [Fuca 1-* 2] Gal/31->-R;然而，该决定簇由表I中列出的大量核心结构携带[Watkins（1980）和Hakomori（1981）综述]。近年来，随着单克隆抗体技术的发展，血型的复杂性和变异性
Program of Biochemical Oncology/Membrane Research, Fred Hutchinson Cancer Research Center, and Departments of Pathobiology, Microbiology, and Immunology, University of Washington, Seattle, Washington 98104, and Department of Biochemistry, University of Alabama at Birmingham, Birmingham, Alabama 35294 Received April 12, 1985 abstract: Three hybridomas secreting monoclonal antibodies, HH1, HH2, and HH3, defining different difucosyl A structures (ALeb or ALey), have been established. Antibody HH1 (IgG2a) reacts specifically with the difucosyl A structure irrespective of a type 1 or type 2 chain, while antibody HH2 (IgG3) reacts exclusively with the difucosyl type 2 chain A (ALey) and does not react with the difucosyl type 1 chain or monofucosyl type 2 chain. Antibody HH3 (IgG2a) reacts exclusively with the difucosyl type 1 chain A (ALeb) and does not react with the monofucosyl type 1 chain A or mono- and difucosyl type 2 chain A. These hybridoma antibodies were obtained by immunization of mice with purified glycolipid antigens and were selected by their reactivity with the specific glycolipid structures. These antibodies, together with previously established monoclonal antibody AH-21, specific for monofucosyl type 1 chain A, and monoclonal antibody TH-1, specific for type 3 chain A, are extremely usefulto define blood group A variants present in cells and tissues. e blood group A determinant is a well-established tri-saccharide, GalN Aca 1—3 [Fuca 1-* 2] Gal/31—>-R; however, the determinant is carried by a large number of core structures as listed in Table I [reviewed by Watkins (1980) and Hakomori (1981)]. With therecent development of the monoclonal antibody approach, the complexity and variation in blood group