Effect of Fixation with Reduced Osmium Tetroxide upon the Antigenicity of Liver Catalase and Erythrocyte Esterase D
Effect of Fixation with Reduced Osmium Tetroxide upon the Antigenicity of Liver Catalase and Erythrocyte Esterase D
复制标题
还原型四氧化锇固定对肝过氧化氢酶和红细胞酯酶 D 抗原性的影响
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Yoshiie Okada
中科院分区：
文献类型：
--
作者：
S. Yokota;Yoshiie Okada
研究了减少OsO4固定对大鼠肝脏过氧化氢酶和红细胞酯酶D抗原性的影响。用4%多聚甲醛和0.2%戊二醛的混合物固定后，用0.1% ~ 1.0%的还原锇固定大鼠肝脏和红细胞1小时。部分红细胞用0.5%还原锇固定不同时间（10min ~ 60min）。两种酶的抗原性以标记密度（过氧化物酶体或红细胞的金粒/μm2）表示。还原OsO4的固定极大地影响了这两种酶的抗原性。当OsO4降低0.4%时，过氧化氢酶的抗原性损失约80%，而当OsO4降低0.2%时，酯酶D的抗原性损失约80%。0.6% OsO4时膜结构明显呈阳性对比。在OsO4浓度降低0.5%的情况下，固定后10min，酯酶D抗原性损失约80%。之后，抗原性下降非常缓慢。结果表明，在还原OsO4浓度下，过氧化氢酶和酯酶D的抗原性丧失80%。
Effect of reduced OsO4 fixation on the antigenicity of rat liver catalase and erythrocyte esterase D was investigated. After fixation with mixture of 4% paraformaldehyde and 0.2% glutaraldehyde, rat liver and erythrocytes were post-fixed with reduced osmium (0.1% to 1.0%) for 1 hr. Some erythrocytes were fixed with 0.5% reduced osmium for various times (10 min to 60min). The antigenicity of both enzymes was expressed as labeling density (gold particles/μm2 of peroxisomes or erythrocytes). Fixation with reduced OsO4 greatly affected the antigenicity of both enzymes. Approximately 80% of catalase antigenicity was lost at 0.4% reduced OsO4, whereas about 80% of esterase D antigenicity was lost at 0.2%. Positive contrast of membrane structures clearly appeared at 0.6% OsO4. About 80% loss of the esterase D antigenicity was observed at 10min after fixation with 0.5% reduced OsO4. Afterwards, the antigenicity decreased very slowly. The data indicates that at the concentration of reduced OsO4 which stains the membrane structures positive contrast, 80% of the antigenicity is lost in the cases of catalase and esterase D.