Vacuolar protein sorting receptor in Schizosaccharomyces pombe
Vacuolar protein sorting receptor in Schizosaccharomyces pombe
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DOI：
10.1099/mic.0.28627-0
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发表时间：
2006-05-01
期刊：
影响因子：
2.8
通讯作者：
Takegawa, Kaoru
中科院分区：
文献类型：
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作者：
Iwaki, Tomoko;Hosomi, Akira;Takegawa, Kaoru
在酿酒酵母中，可溶性蛋白质如羧基肽酶Y到达液泡的机制与哺乳动物细胞中溶酶体蛋白分选的机制非常相似。Vps10p是酿酒酵母中运输可溶性空泡蛋白的受体。Vps10(+)是一个编码酿酒酵母PEP1/VPS10同源物的基因，已从裂殖酵母中发现并缺失。Vps10(+)基因缺失导致SCH错误分选和分泌。Pombe液泡羧肽酶Cpy1p，表明它是将Cpy1p靶向液泡所必需的。Sch.Pombe Vps10p(SpVps10p)是一种I型跨膜蛋白，其C端胞质尾区是Cpy1p转运到液泡中所必需的。表达绿色荧光蛋白标记的SpVps10p的细胞产生了点状的荧光，表明SpVps10p主要定位于高尔基体。此外，Sch.鉴定并缺失了编码酿酒酵母反转录组分VPS26、VPS29和VPS35同源基因的pombe vps26(+)、vps29(+)和VPS35(+)。荧光显微镜观察表明，SpVps10p错误地定位于这些突变体的液泡膜上。这些结果表明，Sch的SpVps10p需要vps26(+)、vps29(+)和VPS35(+)基因产物从泡前室逆行运输回高尔基体。庞贝细胞。
The mechanism by which soluble proteins, such as carboxypeptidase Y, reach the vacuole in Saccharomyces cerevisiae is very similar to the mechanism of lysosomal protein sorting in mammalian cells. Vps10p is a receptor for transport of soluble vacuolar proteins in S. cerevisiae. vps10(+), a gene encoding a homologue of S. cerevisiae PEP1/VPS10, has been identified and deleted from the fission yeast Schizosaccharomyces pombe. Deletion of the vps10(+) gene resulted in missorting and secretion of Sch. pombe vacuolar carboxypeptidase Cpy1p, indicating that it is required for targeting Cpy1 p to the vacuole. Sch. pombe Vps10p (SpVps10p) is a type I transmembrane protein and its C-terminal cytoplasmic tail domain is essential for Cpy1p p transport to the vacuole. Cells expressing green fluorescent protein-tagged SpVps10p produced a punctate pattern of fluorescence, indicating that SpVps10p was largely localized in the Golgi compartment. In addition, Sch. pombe vps26(+), vps29(+) and vps35(+), encoding homologues of the S. cerevisiae retromer components VPS26, VPS29 and VPS35, were identified and deleted. Fluorescence microscopy demonstrated that SpVps10p mislocalized to the vacuolar membrane in these mutants. These results indicate that the vps26(+), vps29(+) and vps35(+) gene products are required for retrograde transport of SpVps10p from the prevacuolar compartment back to the Golgi in Sch. pombe cells.