DNA-SEQUENCE OF THE CARA GENE AND THE CONTROL REGION OF CARAB - TANDEM PROMOTERS, RESPECTIVELY CONTROLLED BY ARGININE AND THE PYRIMIDINES, REGULATE THE SYNTHESIS OF CARBAMOYL-PHOSPHATE SYNTHETASE IN ESCHERICHIA-COLI K-12
DNA-SEQUENCE OF THE CARA GENE AND THE CONTROL REGION OF CARAB - TANDEM PROMOTERS, RESPECTIVELY CONTROLLED BY ARGININE AND THE PYRIMIDINES, REGULATE THE SYNTHESIS OF CARBAMOYL-PHOSPHATE SYNTHETASE IN ESCHERICHIA-COLI K-12
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DOI：
10.1073/pnas.81.13.4134
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
PIERARD, A
中科院分区：
文献类型：
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作者：
PIETTE, J;NYUNOYA, H;PIERARD, A
对E.大肠杆菌K-12编码氨甲酰磷酸合成酶（谷氨酰胺水解）[CO2：L-谷氨酰胺酰胺连接酶（ADP形成，氨基甲酸盐磷酸化）; EC 6.3.5.5]的2个亚基，其被精氨酸和嘧啶累积抑制。描述了carAB的控制区的结构和carA基因的序列。核酸酶S1作图实验表明，carAB控制区内的2个相邻的串联启动子作为起始位点。上游启动子P1受嘧啶调控，下游启动子P2受精氨酸调控。衰减控制似乎不参与carAB的表达。一个可能的机制，控制在这些启动子并发产生的累积模式的镇压进行了讨论。carA的翻译起始是非典型的;它由UUG或AUU密码子组成。
The carAB operon of E. coli K-12, which encodes the 2 subunits of carbamoyl-phosphate synthetase (glutamine hydrolyzing) [CO2: L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating); EC 6.3.5.5], is cumulatively repressed by arginine and the pyrmidines. The structure of the control region of carAB and the sequence of the carA gene are described. Nuclease S1 mapping experiments show that 2 adjacent tandem promoters within the carAB control region serve as initiation sites. The upstream promoter P1 is controlled by pyrimidines; the downstrean promoter P2 is regulated by arginine. Attenuation control does not appear to be involved in the expression of carAB. A possible mechanism by which control at these promoters concurs to produce a cumulative pattern of repression is discussed. The translational start of carA is atypical; it consists of a UUG or AUU codon.