An efficient and inexpensive method for measuring long-term thermoregulatory behavior.

An efficient and inexpensive method for measuring long-term thermoregulatory behavior.
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一种测量长期体温调节行为的高效且廉价的方法。

DOI:
10.1016/j.jtherbio.2016.07.016
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发表时间:
2016
影响因子:
2.7
通讯作者:
Rohr,JasonR
Rohr,JasonR
中科院分区:
生物学3区
文献类型:
--
作者:
Sauer,ErinL;Sperry,JinelleH;Rohr,JasonR

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体温调节能力和行为影响有机体对其环境的反应。通过测量热偏好,研究人员可以更好地了解温度耐受性对生物和非生物应激源的生态和生理反应的影响。然而,由于资金限制和混杂因素,测量体温调节通常很困难。在这里,我们提供了一种有效、价格实惠(每单位约 50 美元)、易于构建且经过验证的设备,用于测量动物的长期热偏好。在测试中,设备的温度范围为 9.29 至 33.94 °C,我们提供了进一步扩大该范围的方法。此外,我们提供简单的方法来非侵入性地测量动物和基质温度,并防止焦点生物的温度偏好与其猎物的温度偏好及其湿度偏好相混淆。为了验证该装置,我们证明它能够在三周内检测南方蟾蜍(Anaxyrusterrestris)和古巴树蛙(Osteopilus septentrionalis)偏好体温的个体水平一致性和个体水平差异。我们设计的几乎每个方面都可以适应多种研究系统的需求,包括各种陆地两栖和水生生物。这里描述的装置和方法可用于量化行为热偏好,这对于确定物种之间的温度耐受性以及物种对当前和即将发生的气候变化的弹性至关重要。
Thermoregulatory ability and behavior influence organismal responses to their environment. By measuring thermal preferences, researchers can better understand the effects that temperature tolerances have on ecological and physiological responses to both biotic and abiotic stressors. However, because of funding limitations and confounders, measuring thermoregulation can often be difficult. Here, we provide an effective, affordable (~$50 USD per unit), easy to construct, and validated apparatus for measuring the long-term thermal preferences of animals. In tests, the apparatus spanned temperatures from 9.29 to 33.94 °C, and we provide methods to further increase this range. Additionally, we provide simple methods to non-invasively measure animal and substrate temperatures and to prevent temperature preferences of the focal organisms from being confounded with temperature preferences of its prey and its humidity preferences. To validate the apparatus, we show that it was capable of detecting individual-level consistency and among individual-level variation in the preferred body temperatures of Southern toads (Anaxyrusterrestris) and Cuban tree frogs (Osteopilus septentrionalis) over three-weeks. Nearly every aspect of our design is adaptable to meet the needs of a multitude of study systems, including various terrestrial amphibious, and aquatic organisms. The apparatus and methods described here can be used to quantify behavioral thermal preferences, which can be critical for determining temperature tolerances across species and thus the resiliency of species to current and impending climate change.
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