Probing Labdane-Related Diterpenoid Biosynthesis in the Fungal Genus Aspergillus.
Probing Labdane-Related Diterpenoid Biosynthesis in the Fungal Genus Aspergillus.
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探索曲霉属真菌中与 Labdane 相关的二萜生物合成。
DOI：
10.1021/acs.jnatprod.6b00764
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发表时间：
2017-02-24
影响因子：
5.1
通讯作者：
Peters RJ
中科院分区：
文献类型：
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作者：
Xu M;Hillwig ML;Tiernan MS;Peters RJ
虽然萜类化合物的产生通常与植物有关，但多种真菌含有预测导致这种生物合成的操纵子。值得注意的是，真菌含有大量的环化酶的特征劳丹相关的二萜类代谢，这还没有太多的探索。这些也经常被发现附近的细胞色素P450（α）单加氧酶，推测进一步装饰随之而来的二萜，这表明这些真菌可能会产生更复杂的二萜类化合物。为了探索这种生物合成能力的功能多样性，进行了来自真菌属曲霉的遗传多样性环化酶和相关CYP的研究，揭示了它们产生异海松二烯衍生的二萜类化合物的能力。有趣的是，劳丹相关的二萜类生物合成基因主要存在于植物相关真菌中，暗示这些天然产物可能在这种相互作用中发挥作用。因此，这里假设异海松烷的产生可能有助于曲霉菌属真菌的植物-腐殖生活方式。
While terpenoid production is generally associated with plants, a variety of fungi contain operons predicted to lead to such biosynthesis. Notably, fungi contain a number of cyclases characteristic of labdane-related diterpenoid metabolism, which have not been much explored. These also are often found near cytochrome P450 (CYP) mono-oxygenases that presumably further decorate the ensuing diterpene, suggesting that these fungi might produce more elaborate diterpenoids. To probe the functional diversity of such biosynthetic capacity, an investigation of the phylogenetically diverse cyclases and associated CYPs from the fungal genus Aspergillus was undertaken, revealing their ability to produce isopimaradiene-derived diterpenoids. Intriguingly, labdane-related diterpenoid biosynthetic genes are largely found in plant-associated fungi, hinting that these natural products may play a role in such interactions. Accordingly, it is hypothesized here that isopimarane production may assist the plant-saprophytic lifestyle of Aspergillus fungi.