Gastroprotective mechanism of Bauhinia thonningii Schum
Gastroprotective mechanism of Bauhinia thonningii Schum
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DOI：
10.1016/j.jep.2013.04.027
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发表时间：
2013-06-21
影响因子：
5.4
通讯作者：
Hashim, Onn Haji
中科院分区：
文献类型：
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作者：
Abdelwahab, Siddig Ibrahim;Taha, Manal Mohamed Elhassan;Hashim, Onn Haji
民族药理学相关性：Bauhinia thonningii Schum。 （Cesalpiniaceae）在当地被称为 Tambarib，用于治疗包括胃溃疡在内的多种疾病。研究目的：本研究旨在评估紫荆叶的甲醇（MEBT）和氯仿（CEBT）提取物对乙醇引起的胃溃疡的胃保护机制。材料和方法：胃酸度、粘液的定量和组织化学、肉眼和显微镜检查、一氧化氮、脂质利用过氧化、2D 凝胶电泳、质谱和生化测试来评估 MEBT 和 CEBT 胃保护作用的机制。这些提取物对脂多糖/干扰素-γ刺激的啮齿动物细胞的影响是在体外进行的。还进行了体外和体内毒性研究。使用 DPPH、FRAP 和 ORAC 测定法检查 MEBT 和 CEBT 的抗氧化活性。使用化学和光谱方法对 MEBT 和 CEBT 进行植物化学分析。结果：大体和组织学特征证实了紫荆的抗溃疡特性。据观察，MEBT 的胃保护机制是通过调节 PAS 反应物质、MDA 和蛋白质组生物标志物（肌酸激酶、苹果酸脱氢酶、ATP 合成酶、肌动蛋白和硫氧还蛋白）来介导的。 MEBT和CEBT在体外和体内对一氧化氮没有显着影响。与 CEBT 相比，甲醇提取物 (MEBT) 显示出优异的胃保护作用、多酚含量和抗氧化活性。植物提取物没有表现出体外或体内毒性。结论：可以得出结论，MEBT具有抗溃疡活性，这可能归因于抑制乙醇诱导的氧化损伤和干预蛋白质组途径，而不是一氧化氮途径。 (C) 2013 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Bauhinia thonningii Schum. (Cesalpiniaceae) is locally known as Tambarib and used to treat various diseases including gastric ulcer.Aim of the study: The current study aims to evaluate the gastroprotecive mechanism(s) of methanolic (MEBT) and chloroform (CEBT) extracts of Bauhinia thonningii leaves on ethanol-induced gastric ulceration.Materials and methods: Gastric acidity, quantification and histochemistry of mucus, gross and microscopic examination, nitric oxide, lipid peroxidation, 2D gel electrophoresis, mass spectroscopy and biochemical tests were utilized to assess the mechanism(s) underlying the gastroprotective effects of MEBT and CEBT. Effect of these extracts into lipopolysaccharide/interferon-gamma stimulated rodent cells were done in vitro. In vitro and in vivo toxicity studies were also conducted. Antioxidant activities of MEBT and CEBT were examined using DPPH, FRAP and ORAC assays. Phytochemical analyses of MEBT and CEBT were conducted using chemical and spectroscopic methods.Results: Gross and histological features confirmed the anti-ulcerogenic properties of Bauhinia thonningii. Gastroprotective mechanism of MEBT was observed to be mediated through the modulation of PAS-reactive substances, MDA and proteomics biomarkers (creatine kinase, malate dehydrogenase, ATP synthase, actin and thioredoxin). MEBT and CEBT showed no significant in vitro and in vivo effects on nitric oxide. Methanolic extract (MEBT) showed superior gastroprotective effects, polyphenolic content and antioxidant activities compared to CEBT. The plant extracts showed no in vitro or in vivo toxicity.Conclusion: It could be concluded that MEBT possesses anti-ulcer activity, which could be attributed to the inhibition of ethanol-induced oxidative damage and the intervention in proteomic pathways but not the nitric oxide pathway. (C) 2013 Elsevier Ireland Ltd. All rights reserved.