Development and application of an UHPLC-MS method for comparative pharmacokinetic study of phenolic components from dragon's blood in rats under simulated microgravity environment.
Development and application of an UHPLC-MS method for comparative pharmacokinetic study of phenolic components from dragon's blood in rats under simulated microgravity environment.
复制标题
DOI：
10.1016/j.jpba.2016.01.014
复制
发表时间：
2016-03
影响因子：
3.4
通讯作者：
Yujuan Li;George Q. Li;Yongzhi Li;Yulin Deng;L. Deng
中科院分区：
文献类型：
--
作者：
Yujuan Li;George Q. Li;Yongzhi Li;Yulin Deng;L. Deng
龙血是一种常用的中草药，在模拟微重力条件下对大鼠和小鼠具有保护作用。本研究旨在建立一种超高效液相色谱串联质谱（UHPLC-MS）方法，同时测定大鼠体内龙血素A、龙血素C、7,4′-二羟基黄酮和紫檀芪等4种酚类成分的含量，并利用该方法对模拟微重力环境下口服龙血竭后大鼠体内这些成分的药动学（PK）和排泄情况进行比较研究。结果表明，所开发的 UHPLC-MS 方法灵敏且快速。大鼠药代动力学比较研究表明，龙血素A、龙血素C和7,4-二羟基黄酮在模拟微重力环境下，可降低Cmax和AUC，增加Vd和CL；而紫檀芪却有相反的变化。在模拟微重力大鼠中，四种酚类成分的排泄量也显示出增加或减少。这些结果表明化学结构和理化性质以及生理条件可能对模拟微重力环境中酚类成分的吸收和排泄产生影响。它还意味着不同的药物在相同的航天条件下可能表现不同，导致药效结果的增加或减少。
Dragon’s blood is a commonly used Chinese herbal medicine shown to have protective effects in simulated microgravity in rats and mice. The current study aimed to develop an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS) method for simultaneous determination of four phenolic components from the herb: loureirin A, loureirin C, 7,4′-dihydroxyflavone and pterostilbene in rats, and use the method for comparative study on the pharmacokinetics (PK) and excretion of these components in rats after oral dosage of dragon’s blood under simulated microgravity environments. The results showed the developed UHPLC–MS method was sensitive and rapid. The comparative pharmacokinetic study in rats showed loureirin A, loureirin C and 7,4-dihydroxyflavone had decreasedCmaxand AUC and increasedVdand CL in simulated microgravity environment; but pterostilbene had the opposite changes. The four phenolic components also showed increased or decreased excretions in simulated microgravity rats. These results indicate the chemical structure and physicochemical property, as well as physiological conditions may have an impact on the absorption and excretion of phenolic components in simulated microgravity environment. It also implies that different drug may behave differently in the same spaceflight condition leading to an increase or a reduction in pharmacodynamic outcomes.