Gene expression profiling of osteoclast differentiation by combined suppression subtractive hybridization (SSH) and cDNA microarray analysis
Gene expression profiling of osteoclast differentiation by combined suppression subtractive hybridization (SSH) and cDNA microarray analysis
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DOI：
10.1089/104454902320308915
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发表时间：
2002-08-01
影响因子：
3.1
通讯作者：
Choi, Y
中科院分区：
文献类型：
--
作者：
Rho, J;Altman, CR;Choi, Y
骨稳态通过骨形成成骨细胞和骨吸收破骨细胞的平衡作用来维持。多核、成熟的破骨细胞由造血干细胞通过单核细胞-巨噬细胞谱系发育而成，同时也产生巨噬细胞和树突状细胞。尽管这些细胞类型在骨骼和免疫系统中具有独特的生理作用，但它们具有许多分子和生化特征。为了深入了解破骨细胞如何分化和发挥控制骨代谢的作用，我们采用了一种系统方法，通过结合抑制消减杂交 (SSH) 和 cDNA 微阵列分析来分析破骨细胞特异性基因表达模式。在这里，我们研究了成熟破骨细胞的基因表达谱与巨噬细胞或树突状细胞的基因表达谱有何不同，破骨细胞分化过程中基因表达谱如何变化，以及对破骨细胞成熟至关重要的转录因子 Mitf 如何影响基因表达谱。这种方法揭示了一组协调调节破骨细胞功能的基因，其中一些基因以前曾与人类的几种骨骼疾病有关。
Bone homeostasis is maintained by the balanced action of bone-forming osteoblasts and bone-resorbing osteoclasts. Multinucleated, mature osteoclasts develop from hematopoietic stem cells via the monocyte-macrophage lineage, which also give rise to macrophages and dendritic cells. Despite their distinct physiologic roles in bone and the immune system, these cell types share many molecular and biochemical features. To provide insights into how osteoclasts differentiate and function to control bone metabolism, we employed a systematic approach to profile patterns of osteoclast-specific gene expression by combining suppression subtractive hybridization (SSH) and cDNA microarray analysis. Here we examined how gene expression profiles of mature osteoclast differ from macrophage or dendritic cells, how gene expression profiles change during osteoclast differentiation, and how Mitf, a transcription factor critical for osteoclast maturation, affects the gene expression profile. This approach revealed a set of genes coordinately regulated for osteoclast function, some of which have previously been implicated in several bone diseases in humans.