Spot the difference: applications of subtractive hybridisation to the study of bacterial pathogens.
Spot the difference: applications of subtractive hybridisation to the study of bacterial pathogens.
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DOI：
10.1099/0022-1317-51-6-459
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发表时间：
2002-06
影响因子：
3
通讯作者：
C. Winstanley
中科院分区：
文献类型：
--
作者：
C. Winstanley
将来自强毒菌株的细菌病原体的DNA与来自弱毒或无毒近亲的DNA进行比较，可以鉴定出只存在于强毒菌株中的那些基因组区域。这些区域通常与致病性岛屿有关，它们的特征可以使人们更好地了解传染病的发病机制。现在有一个庞大的细菌基因组序列数据库，可以提供有用的参考信息，用于比较在毒力或宿主偏好方面表现出变化的菌株的基因组。减法杂交（SH）和它的姊妹方法，抑制减法杂交（SSH），是设计用于识别在一个基因组中存在但在另一个基因组中缺失的区域的技术。这些技术的应用已经导致了一系列细菌病原体中pi，移动遗传元件和毒力基因表达变异的鉴定。
Comparison of DNA from virulent strains of bacterial pathogens with DNA from less virulent or avirulent close relatives allows the identification of those genomic regions that are present only in virulent strains. Such regions are often associated with pathogenicity islands (PIs) and their characterisation can lead to a greater understanding of the pathogenesis of infectious diseases. There is now a large database of bacterial genomic sequences that provides useful reference information with which to compare the genomes of strains that exhibit variations in virulence or host preferences. Subtractive hybridisation (SH) and its sister method, suppression subtractive hybridisation (SSH), are techniques designed to identify those regions present in one genome but absent from another. The application of these techniques has led to the identification of PIs, mobile genetic elements and variations in virulence gene expression in a range of bacterial pathogens.