The anti-inflammatory and antioxidant effects of bergamot juice extract (BJe) in an experimental model of inflammatory bowel disease
The anti-inflammatory and antioxidant effects of bergamot juice extract (BJe) in an experimental model of inflammatory bowel disease
复制标题
DOI：
10.1016/j.clnu.2014.11.012
复制
发表时间：
2015-12-01
影响因子：
6.3
通讯作者：
Navarra, Michele
中科院分区：
文献类型：
--
作者：
Impellizzeri, Daniela;Bruschetta, Giuseppe;Navarra, Michele
背景与目的：佛手柑汁（BJe）中黄酮类成分的有益特性已引起人们的兴趣，并已成为最近研究的主题，考虑到其促进健康的物质的潜力。黄酮类化合物具有清除自由基和抗炎活性。本研究的目的是检查BJe对实验性结肠炎小鼠的影响。方法：用二硝基苯磺酸（DNBS）灌胃诱导小鼠结肠炎。Bje每日口服（5、10和20 mg/kg）。结果：DNBS给药4 d后，结肠核因子nf - κ B易位、MAP激酶磷酸化- jnk活化增加，肿瘤坏死因子(TNF)- α等细胞因子产生增加。和白细胞介素-1 β。中性粒细胞通过髓过氧化物酶（MPO）活性在粘膜中的浸润与黏附分子（ICAM-1和p-选择素）的上调有关。免疫组化检测硝基酪氨酸和聚adp核糖（PAR）在炎症结肠中也显示强烈染色。BJe治疗减少了腹泻的出现和体重减轻。这与结肠MPO活性降低有关。BJe降低核NF-kappa B易位、p-JNK活化、促炎细胞因子释放、硝基酪氨酸和PAR在结肠中的出现，并降低ICAM-1和p-选择素的上调。此外，结肠炎症也与细胞凋亡损伤有关。BJe处理导致促凋亡Bax表达降低，抗凋亡Bc1-2表达升高。结论：本研究的结果表明，BJe诱导了部分指定的抗炎机制，这可能对人类IBD的治疗有益。(C) 2014爱思唯尔有限公司和欧洲临床营养与代谢学会。版权所有。
Background & aims: The beneficial properties of the flavonoid fraction of bergamot juice (BJe) have been raising interest and have been the subject of recent studies, considering the potentiality of its health promoting substances. Flavonoids have demonstrated radical-scavenging and anti-inflammatory activities. The aim of the present study was to examine the effects of BJe in mice subjected to experimental colitis.Methods: Colitis was induced in mice by intracolonic instillation of dinitrobenzene sulfonic acid (DNBS). Bje was administered daily orally (at 5, 10 and 20 mg/kg).Results: Four days after DNBS administration, colon nuclear factor NF-kappa B translocation and MAP kinase phospho-JNK activation were increased as well as cytokine production such as tumor necrosis factor (TNF)-alpha. and interleukin (IL)-1 beta. Neutrophil infiltration, by myeloperoxidase (MPO) activity, in the mucosa was associated with up-regulation of adhesion molecules (ICAM-1 and P-selectin). Immunohistochemistry for nitrotyrosine and poly ADP-ribose (PAR) also showed an intense staining in the inflamed colon. Treatment with BJe decreased the appearance of diarrhea and body weight loss. This was associated with a reduction in colonic MPO activity. BJe reduced nuclear NF-kappa B translocation, p-JNK activation, the pro-inflammatory cytokines release, the appearance of nitrotyrosine and PAR in the colon and reduced the up-regulation of ICAM-1 and P-selectin. In addition, colon inflammation was also associated with apoptotic damage. Treatment with BJe caused a decrease of pro-apoptotic Bax expression and an increase of anti-apoptotic Bc1-2 expression.Conclusions: The results of this study suggested that administration of BJe induced, partly specified, antiinflammatory mechanisms, which potentially may be beneficial for the treatment of IBD in humans. (C) 2014 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.