Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination
Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination
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DOI：
10.1364/ol.32.002846
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发表时间：
2007-10-01
期刊：
影响因子：
3.6
通讯作者：
Mertz, Jerome
中科院分区：
文献类型：
--
作者：
Chu, Kengyeh K.;Lim, Daryl;Mertz, Jerome
我们描述了一种技术，以提高弱信号的相对灵敏度和动态范围的激光扫描光学显微镜。该技术是基于维持一个固定的检测功率的照明功率的快速反馈控制，从而将高测量分辨率弱信号，同时几乎消除了图像饱和的可能性。我们分析并证明了自适应照明在双光子荧光显微镜的好处。(c)2007年，美国光学学会。
We describe a technique to enhance both the weak-signal relative sensitivity and the dynamic range of a laser scanning optical microscope. The technique is based on maintaining a fixed detection power by fast feedback control of the illumination power, thereby transferring high measurement resolution to weak signals while virtually eliminating the possibility of image saturation. We analyze and demonstrate the benefits of adaptive illumination in two-photon fluorescence microscopy. (c) 2007 Optical Society of America.