Primary nitrate responses via calcium signalling and diverse protein phosphorylation.
Primary nitrate responses via calcium signalling and diverse protein phosphorylation.
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通过钙信号传导和多种蛋白质磷酸化的初级硝酸盐反应。
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Jen Sheen
中科院分区：
文献类型：
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作者：
Kun-Hsiang Liu;Andrew Diener;Ziwei Lin;Cong Liu;Jen Sheen
硝酸盐是植物无机氮的主要来源，是控制植物体内营养物质运输、同化和适应性生长反应的重要信号。了解植物如何感知硝酸盐，以及这种感知如何转化为优化生长的反应，对于合理提高作物生产力和减轻使用化肥造成的污染非常重要。这篇综述强调了最近的研究结果，揭示了通过不同的机制在初级硝酸盐反应（PNR）的细胞核钙信号和动态蛋白磷酸化的关键作用。硝酸盐触发的钙签名，以及亚组III钙传感器蛋白激酶，一个特定的蛋白磷酸酶2C和RNA聚合酶II C-末端结构域磷酸酶样3的关键功能进行了讨论。此外，硝酸盐调节基因编码候选蛋白激酶和磷酸酶调节关键磷酸化事件在PNR的基因组荟萃分析阐述。我们还考虑如何磷酸化蛋白质组学的方法可以有助于确定推定的调节蛋白激酶的PNR。探索和整合实验策略，新方法和全面的数据集将进一步推进我们对PNR复杂调控过程的分子和细胞机制的理解。
Nitrate, the major source of inorganic nitrogen to plants, is a critical signal controlling nutrient transport, assimilation and adaptive growth responses throughout the plant. Understanding how plants perceive nitrate and how this perception is transduced into responses that optimize growth are important for the rational improvement of crop productivity and mitigation of pollution from use of fertilizers. This review highlights recent findings that reveal key roles of cytosolic-nuclear calcium signalling and dynamic protein phosphorylation via diverse mechanisms in the primary nitrate responses (PNR). Nitrate-triggered calcium signatures as well as the critical functions of subgroup III calcium-sensor protein kinases, a specific protein phosphatase 2C, and RNA polymerase II C-terminal domain phosphatase-like 3 are discussed. Moreover, genome-wide meta-analysis of nitrate-regulated genes encoding candidate protein kinases and phosphatases for modulating critical phosphorylation events in the PNR are elaborated. We also consider how phosphoproteomics approaches can contribute to the identification of putative regulatory protein kinases in the PNR. Exploring and integrating experimental strategies, new methodologies, and comprehensive datasets will further advance our understanding of the molecular and cellular mechanisms underlying the complex regulatory processes in the PNR.