Assessing genetic diversity of Elymus sibiricus (Poaceae: Triticeae) populations from Qinghai-Tibet Plateau by ISSR markers
Assessing genetic diversity of Elymus sibiricus (Poaceae: Triticeae) populations from Qinghai-Tibet Plateau by ISSR markers
复制标题
利用ISSR标记评价青藏高原披碱草（禾本科：小麦科）种群遗传多样性
DOI：
10.1016/j.bse.2008.03.003
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发表时间：
2008-07-01
影响因子：
1.6
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Ma, Xiao;Zhang, Xin-Quan;Liu, Wei
利用ISSR分子标记技术对西伯利亚野马8个居群的遗传多样性和居群结构进行了分析。来自中国青藏高原东南部。在筛选的100个引物中，有13个引物扩增出了重复性好的条带。使用这些引物，产生193个可辨别的DNA片段，其中149个（77.2%）是多态性的，表明相当大的遗传变异在物种水平。而在群体水平上的多态性水平相对较低，多态性条带百分率（PPB）在44.04%~ 54.92%之间。种群内平均基因多样性为0.181（范围0.1640-200），种水平平均基因多样性为0.274。根据Nei’s遗传多样性分析（33.1%）、Shannon’s指数分析（34.5%）、Bayesian方法（33.2%）和AMOVA分析（42.5%），群体间的遗传分化程度较高。在不同的样品采集区域之间，没有发现显著的统计学差异（分子方差分析[AMOVA]，P=0.08）在ISSR变异。但居群间（方差的42.5%）和居群内（方差的57.5%）差异显著（P
Inter-simple sequence repeats (ISSR) markers were used to assess the genetic diversity and population structure in eight populations of Elymus sibiricus L. from the southeast of Qinghai-Tibet Plateau of China. Of the 100 primers screened, 13 produced highly reproducible ISSR bands. Using these primers, 193 discernible DNA fragments were generated with 149 (77.2%) being polymorphic, indicating considerable genetic variation at the species level. In contrast, there were relatively low levels of polymorphism at the population level with the percentage of polymorphic bands (PPB) ranging from 44.04 to 54.92%. The mean gene diversity (HE) was estimated to be 0.181 within populations (range 0.1640-200), and 0.274 at the species level. A high level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (33.1%), Shannon's index analysis (34.5%), Bayesian method (33.2%) and AMOVA analysis (42.5%). No significant statistical differences (analysis of molecular variance [AMOVA], P=0.08) in ISSR variation were found between the sample collection regions. However, among populations (42.5% of the variance) and within populations (57.5% of the variance), there were significant differences (P