A HYDROGEN-OXIDIZING, FE(III)-REDUCING MICROORGANISM FROM THE GREAT BAY ESTUARY, NEW-HAMPSHIRE
A HYDROGEN-OXIDIZING, FE(III)-REDUCING MICROORGANISM FROM THE GREAT BAY ESTUARY, NEW-HAMPSHIRE
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DOI：
10.1128/aem.58.10.3211-3216.1992
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发表时间：
1992-10-01
影响因子：
4.4
通讯作者：
LOVLEY, DR
中科院分区：
文献类型：
--
作者：
CACCAVO, F;BLAKEMORE, RP;LOVLEY, DR
从新罕布什尔州大海湾河口的底部沉积物中分离出一种异化的Fe(III)-和Mn(IV)-还原细菌。分离物是兼性厌氧革兰氏阴性棒，似乎不适合任何先前描述的属。它暂时被命名为BrY菌株。BrY在以氢或乳酸盐为电子供体，铁（III）为电子受体的培养基中厌氧生长。BrY需要柠檬酸盐、富马酸盐或苹果酸盐作为碳源在H-2和Fe（III）上生长。以Fe（III）作为唯一的电子受体，BrY代谢氢的最低阈值至少比硫酸盐还原剂纯培养的阈值低60倍。这一发现支持了假设，即当Fe（III）可用时，Fe（III）还原剂可以与硫酸盐还原剂竞争电子供体。以Fe（III）为电子受体，将乳酸不完全氧化为乙酸和二氧化碳。在厌氧条件下，乳酸氧化也与Mn(IV)， U(VI)，富马酸盐，硫代硫酸盐或三甲胺n-氧化物的还原相结合。BrY提供了一个模型，说明通过呼吸性金属还原微生物的酶促金属还原如何有可能促进大海湾河口沉积物中铁和微量金属的动员以及铀的固定化。
A dissimilatory Fe(III)- and Mn(IV)-reducing bacterium was isolated from bottom sediments of the Great Bay estuary, New Hampshire. The isolate was a facultatively anaerobic gram-negative rod which did not appear to fit into any previously described genus. It was temporarily designated strain BrY. BrY grew anaerobically in a defined medium with hydrogen or lactate as the electron donor and Fe(III) as the electron acceptor. BrY required citrate, fumarate, or malate as a carbon source for growth on H-2 and Fe(III). With Fe(III) as the sole electron acceptor, BrY metabolized hydrogen to a minimum threshold at least 60-fold lower than the threshold reported for pure cultures of sulfate reducers. This finding supports the hypothesis that when Fe(III) is available, Fe(III) reducers can outcompete sulfate reducers for electron donors. Lactate was incompletely oxidized to acetate and carbon dioxide with Fe(III) as the electron acceptor. Lactate oxidation was also coupled to the reduction of Mn(IV), U(VI), fumarate, thiosulfate, or trimethylamine n-oxide under anaerobic conditions. BrY provides a model for how enzymatic metal reduction by respiratory metal-reducing microorganisms has the potential to contribute to the mobilization of iron and trace metals and to the immobilization of uranium in sediments of Great Bay Estuary.