Femtosecond laser treatment enhances DNA transfection efficiency in vivo.
Femtosecond laser treatment enhances DNA transfection efficiency in vivo.
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DOI：
10.1186/1423-0127-16-36
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发表时间：
2009-04-01
影响因子：
11
通讯作者：
Wu TC
中科院分区：
文献类型：
--
作者：
Tsen SW;Wu CY;Meneshian A;Pai SI;Hung CF;Wu TC
质粒DNA的基因治疗正在成为治疗许多疾病的一种有前途的策略。这种治疗的主要障碍之一是DNA在体内的转染效率差。在这份报告中，我们采用了一个非常低的功率，近红外飞秒激光技术，以提高转染效率的皮内和瘤内注射的DNA质粒。我们发现，飞秒激光治疗可以显着增强DNA进入皮肤和小鼠体内已建立的肿瘤的递送。此外，我们发现激光功率密度以及激光处理的持续时间是增加DNA转染效率的关键参数。飞秒激光技术采用相对不聚焦的激光束，其最大化转染区域，最小化对组织的损伤并简化其实施。这种飞秒激光新技术代表了一种安全和创新的技术，用于增强体内DNA基因转移。
Gene therapy with plasmid DNA is emerging as a promising strategy for the treatment of many diseases. One of the major obstacles to such therapy is the poor transfection efficiency of DNA in vivo. In this report, we employed a very low power, near-infrared femtosecond laser technique to enhance the transfection efficiency of intradermally and intratumorally administered DNA plasmid. We found that femtosecond laser treatment can significantly enhance the delivery of DNA into the skin and into established tumors in mice. In addition, we found that both laser power density as well as duration of laser treatment are critical parameters for augmenting DNA transfection efficiency. The femtosecond laser technique employs a relatively unfocused laser beam that maximizes the transfected area, minimizes damage to tissue and simplifies its implementation. This femtosecond new laser technology represents a safe and innovative technology for enhancing DNA gene transfer in vivo.
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影响因子：
4.8
作者：
Zeira, Evelyne;Manevitch, Alexandra;Galun, Eithan
通讯作者：
Galun, Eithan
影响因子：
5.1
作者：
Hung, C-F;Tsai, Y-C;Wu, T-C
通讯作者：
Wu, T-C
DOI：
10.1159/000125614
发表时间：
2008-01-01
期刊：
GENE THERAPY FOR RENAL DISEASES AND TRANSPLANTATION
影响因子：
--
作者：
Torras, Juan;Cruzado, Josep M.;Grinyo, Josep M.
通讯作者：
Grinyo, Josep M.
影响因子：
1.5
作者：
Cotrim, Ana P.;Baum, Bruce J.
通讯作者：
Baum, Bruce J.
影响因子：
3.1
作者：
Shirakawa, Toshiro;Fujisawa, Masato;Gotoh, Akinobu
通讯作者：
Gotoh, Akinobu